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AB308282

Anti-IgG1 antibody [EPR26761-34] - BSA and Azide free (Capture)

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Anti-IgG1 antibody [EPR26761-34] - BSA and Azide free (Capture) (ab308282) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting IgG1 in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
1 이미지
Sandwich ELISA - Anti-IgG1 antibody [EPR26761-34] - BSA and Azide free (Capture) (AB308282)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IgG1 antibody [EPR26761-34] - BSA and Azide free (Capture) (AB308282)

Sandwich ELISA of ab308281 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26761-34

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IGHG1.

P01857

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보완 제품

Assay Kits

AB195215

Human IgG ELISA Kit

SimpleStep

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

  • 4
  • 6
Sample Prep & Detection Kits

AB201797

Dylight® 350 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Dylight® 350 Conjugation Kit (Fast) - Lightning-Link® (AB201797)

  • 0
  • 0
Sample Prep & Detection Kits

AB186922

Biotin Gold Conjugate (40nm, 10 OD)

Functional Studies - Biotin Gold Conjugate (40nm, 10 OD) (AB186922)

  • 0
  • 0
Primary Antibodies

AB308283

Anti-IgG1 antibody [EPR26761-82] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IgG1 antibody [EPR26761-82] - BSA and Azide free (Detector) (AB308283)

  • 0
  • 0
Primary Antibodies

AB309231

Anti-IgG4 antibody [EPR26764-24] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IgG4 antibody [EPR26764-24] - BSA and Azide free (Detector) (AB309231)

  • 0
  • 0
Primary Antibodies

AB309230

Anti-IgG4 antibody [EPR26764-64] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IgG4 antibody [EPR26764-64] - BSA and Azide free (Capture) (AB309230)

  • 0
  • 0
Primary Antibodies

AB314156

Anti-IgG3 antibody [EPR26763-83] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IgG3 antibody [EPR26763-83] - BSA and Azide free (Detector) (AB314156)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab308282 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human IgG1.
The recommended pair for sandwich ELISA is:
Capture: ab308282, human IgG1 Capture Antibody (unconjugated)
Detector: ab308283, human IgG1 Detector Antibody (unconjugated)
The reference range value is 7.81-1000 pg/ml

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.
See full target information IGHG1

대체 명칭 보기

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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