Anti-IgG4 antibody [RM120]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [RM120] (AB238320)

Formalin-fixed, Paraffin-embedded human lymphoid tissue stained for Human IgG4 using ab238320 at 2 μg/mL in immunohistochemical analysis.

• ELISA

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ELISA - Anti-IgG4 antibody [RM120] (AB238320)

A titer ELISA using ab238320. The plate was coated with different amounts of human IgG4. A serial dilution of ab238320 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.``

• ELISA

Supplier Data

ELISA - Anti-IgG4 antibody [RM120] (AB238320)

Sandwich ELISA using ab238320 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.``

• WB

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Western blot - Anti-IgG4 antibody [RM120] (AB238320)

The Western blot shows ab238320 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG is shown.

All lanes:

Western blot - Anti-IgG4 antibody [RM120] (ab238320) at 2 µg/mL

Lane 1:

non-reduced (-) and reduced (+) forms of Human IgG1

Lane 2:

non-reduced (-) and reduced (+) forms of Human IgG2

Lane 3:

non-reduced (-) and reduced (+) forms of Human IgG3

Lane 4:

non-reduced (-) and reduced (+) forms of Human IgG4

Lane 5:

non-reduced (-) and reduced (+) forms of Mouse IgG

Lane 6:

non-reduced (-) and reduced (+) forms of rat IgG

Lane 7:

non-reduced (-) and reduced (+) forms of goat IgG

Predicted band size: 36 kDa

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• ELISA

Supplier Data

ELISA - Anti-IgG4 antibody [RM120] (AB238320)

ELISA showing ab238320 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of ab238320 and a monkey IgG binding antibody (positive control) was used as the detection antibody.

• ELISA

Supplier Data

ELISA - Anti-IgG4 antibody [RM120] (AB238320)

Detection of human IgG4 in monkey serum. Sandwich ELISA using ab238320 as the capture antibody, and a biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by a HRP conjugated streptavidin.``