Anti-IGJ antibody [SP105] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] - BSA and Azide free (AB240939)

Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) labeling IGJ with purified ab105229 at 1/20 dilution (5.3μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab105229).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (AB240939)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells of human stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature.

This image was generated using ab105229, the same clone, but with a different buffer formulation.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (AB240939)

ab105229, at 1/100 dilution, staining IGJ in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab105229)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (AB240939)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature.

This image was generated using ab105229, the same clone, but with a different buffer formulation.