Anti-IKB beta antibody

• IP

Supplier Data

Immunoprecipitation - Anti-IKB beta antibody (AB264240)

IKB beta was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264240 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264240 at 0.4 μg/ml.

Lane 1 : ab264240 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in Hela whole cell lysate.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-IKB beta antibody (ab264240)

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-IKB beta antibody (AB264240)

All lanes:

Western blot - Anti-IKB beta antibody (ab264240) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 37 kDa

true

Exposure time: 3min