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AB325237

Anti-IL-10RB/IL-10R2 antibody [EPR28963-63]

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Rabbit Recombinant Monoclonal IL-10RB/IL-10R2 antibody. Suitable for WB and reacts with Human, Mouse samples.
3 이미지
Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (AB325237)
  • WB

Lab

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (AB325237)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : cerebellum

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 36308662).

The blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (ab325237) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 80 µg

Lane 2:

Human cerebellum tissue lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 55 kDa,36 kDa

true

Exposure time: 70s

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (AB325237)
  • WB

Lab

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (AB325237)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : SK-N-MC

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 36308662).

The blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (ab325237) at 1/1000 dilution

Lane 1:

A-172 (human brain glioblastoma cell ) whole cell lysate at 80 µg

Lane 2:

SK-N-MC (human brain epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,36 kDa

true

Exposure time: 103s

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (AB325237)
  • WB

Lab

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (AB325237)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : kidney

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 36308662).

To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-10RB/IL-10R2 antibody [EPR28963-63] (ab325237) at 1/1000 dilution

Lane 1:

Mouse lung fresh tissue lysate at 20 µg

Lane 2:

Mouse kidney fresh tissue lysate at 20 µg

Lane 3:

Mouse heart fresh tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28963-63

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB281996

Anti-IL-20R2 antibody [EPR24558-81]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-IL-20R2 antibody [EPR24558-81] (AB281996)

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Primary Antibodies

AB89883

Anti-IL-10RA antibody [MM0359-7Q35]

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Primary Antibodies

AB68153

Anti-STAT3 antibody [EPR787Y]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-STAT3 antibody [EPR787Y] (AB68153)

  • 4
  • 10
Primary Antibodies

AB32520

Anti-STAT6 antibody [YE361]

20ul selling size|Recombinant|Advanced Validation|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (AB32520)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Shared cell surface receptor required for the activation of five class 2 cytokines : IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
See full target information IL10RB

대체 명칭 보기

CDw210b, CRFB4, D21S58, D21S66, IL10RB, Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, CRF2-4, IL-10R subunit 2, IL-10R2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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