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AB214444

Anti-IL-12 p40 antibody [EPR5738] - BSA and Azide free

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(1 출판물)

Rabbit Recombinant Monoclonal IL-12B antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40

2 이미지
Western blot - Anti-IL-12 p40 antibody [EPR5738] - BSA and Azide free (AB214444)
  • WB

Unknown

Western blot - Anti-IL-12 p40 antibody [EPR5738] - BSA and Azide free (AB214444)

This data was developed using ab131156, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IL-12 p40 antibody [EPR5738] (<a href='/ko/products/primary-antibodies/il-12-p40-antibody-epr5738-ab131156'>ab131156</a>) at 1/50000 dilution

All lanes:

Human IL-12 p40 recombinant protein at 0.01 µg

Predicted band size: 37 kDa

false

OI-RD Scanning - Anti-IL-12 p40 antibody [EPR5738] - BSA and Azide free (AB214444)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-IL-12 p40 antibody [EPR5738] - BSA and Azide free (AB214444)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5738

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB220656

Human IL-12/IL-23 p40 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-12/IL-23 p40 ELISA Kit (AB220656)

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Primary Antibodies

AB242971

Anti-IL-23 antibody [EPR19069-180] - BSA and Azide free (Capture)

RabMAb|Recombinant

ELISA - Anti-IL-23 antibody [EPR19069-180] - BSA and Azide free (Capture) (AB242971)

  • 0
  • 0
Assay Kits

AB236717

Mouse IL-12 p40 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

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  • 0
Primary Antibodies

AB66462

Anti-IL-12 p35 antibody

  • 0
  • 0
Assay Kits

AB221837

Human IL-23 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-23 ELISA Kit (AB221837)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

ab214444 is the carrier-free version of ab131156.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
See full target information IL12B
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of investigative dermatology 135:400-410 PubMed25207817

2014

Proteome profiling of human cutaneous leishmaniasis lesion.

Applications

Unspecified application

Species

Unspecified reactive species

Claire da Silva Santos,Sanaz Attarha,Ravi Kanth Saini,Viviane Boaventura,Jackson Costa,Ricardo Khouri,Manoel Barral-Netto,Cláudia Ida Brodskyn,Serhiy Souchelnytskyi
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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