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AB325502

Anti-IL-12RB1 antibody [EPR28354-118]

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Rabbit Recombinant Monoclonal IL-12RB1 antibody. Suitable for IP, WB and reacts with Human samples.
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Immunoprecipitation - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)
  • IP

Lab

Immunoprecipitation - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)

IL-12RB1 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate with ab325502 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325502 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate
Lane 2 : ab325502 IP in HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325502 in HDLM-2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-IL-12RB1 antibody [EPR28354-118] (ab325502)

Lane 1:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 10 µg

Lane 2:

ab325502 at 1/30 IP in HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab325502 in HDLM-2 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)
  • WB

Lab

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (ab325502)

All lanes:

Human tonsil tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)
  • WB

Lab

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : SH-SY5Y, MCF7.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (ab325502)

Lane 1:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 50 µg

Lane 2:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 50 µg

Lane 3:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 50 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 50 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,36 kDa

true

Exposure time: 81s

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)
  • WB

Lab

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (ab325502)

Lane 1:

THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

THP-1 transfected with siRNA specifically targeting IL-12RB1 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,36 kDa

true

Exposure time: 81s

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)
  • WB

Lab

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (AB325502)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-12RB1 antibody [EPR28354-118] (ab325502)

Lane 1:

HDLM-2 (human Hodgkin lymphoma cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

HDLM-2 transfected with siRNA specifically targeting IL-12RB1 whole cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 100 kDa,36 kDa

true

Exposure time: 125s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

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Anti-IL-23R antibody [EPR22838-4]

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  • 0
  • 0
Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

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  • 5
  • 4
Primary Antibodies

AB271042

Anti-IL-6R antibody [EPR24322-143]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

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  • 5
  • 1
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Also associates with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
See full target information IL12RB1

대체 명칭 보기

CD212, IL12R, IL12RB, IL12RB1, Interleukin-12 receptor subunit beta-1, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, IL-12R-beta-1, IL-12RB1, IL-12 receptor beta component
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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