- WB
Supplier Data
Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] (AB322049)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
IL-13 receptor alpha 1 is a glycoprotein of approximately 70 kDa and detected as a 49kDa band after treated with Protein Deglycosylation MIX II.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] (ab322049) at 1/1000 dilution
Lane 1:
Untreated HDLM-2 (human hodgkin lymphoma cell) whole cell lysate at 20 µg
Lane 2:
HDLM-2 whole cell lysate treated with Protein Deglycosylation Mix II at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 70 kDa,49 kDa,36 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] (AB322049)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Negative control : Jurkat (PMID : 15163701), Raji, Ramos (PMID : 11591117).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] (ab322049) at 1/1000 dilution
Lane 1:
PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 30 µg
Lane 2:
Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 30 µg
Lane 3:
Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate at 30 µg
Lane 4:
Ramos (human burkitt's lymphoma b lymphocyte) whole cell lysate at 30 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 70 kDa,36 kDa
false
Exposure time: 114s
관련 conjugated 항체와 다양한 조성의 항체 (1)
-
Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] - BSA and Azide free
Reactivity 정보
제품 세부 정보
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
대체 명칭 보기
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com