Anti-IL-13 receptor alpha 2 antibody [EPR29921-581]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Wild-type A375 cells and IL13RA2 KO A375 cells labelling IL-13 receptor alpha 2 with ab324048 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) antibody at 1/1000 dilution (Green).

Confocal image showing membranous and cytoplasmic staining in parental A375 cell line (shown in green).

The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-alpha Tubulin mouse monoclonal antibody (ab7291) was used to counterstain tubulin at 1/1000 dilution (Magenta).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Flow cytometric analysis of A-172 (human brain glioblastoma cell, Right) / Daudi (human Burkitt's lymphoma lymphoblast, Left) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : Daudi.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Flow cytometric analysis of IL13RA2 KO A375 (IL-13 receptor alpha 2 knock out human malignant melanoma epithelial cell, Magenta) / A375 (Green) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.