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AB242630

Anti-IL-15 antibody [EPR19942-311] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal IL-15 antibody. Carrier free. Suitable for sELISA and reacts with Human samples. Cited in 1 publication.
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Sandwich ELISA - Anti-IL-15 antibody [EPR19942-311] - BSA and Azide free (Capture) (AB242630)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IL-15 antibody [EPR19942-311] - BSA and Azide free (Capture) (AB242630)

Sandwich ELISA - Recombinant human IL-15 protein standard curve Background subtracted standard curve using Recombinant Recombinant Anti-IL-15 antibody [EPR19942-311] - BSA and Azide free (Capture) (ab242630), Recombinant Anti-IL-15 antibody [EPR19942-285] - BSA and Azide free (Detector) (ab242875), and Recombinant human IL-15 protein (Active) (ab259403) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19942-311

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB218266

Human IL-15 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-15 ELISA Kit (AB218266)

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Sample Prep & Detection Kits

AB188286

Rhodamine Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (AB188286)

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Sample Prep & Detection Kits

AB218260

Oligonucleotide Conjugation Kit

Conjugation - Oligonucleotide Conjugation Kit (AB218260)

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Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

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Primary Antibodies

AB271310

Anti-IL-7 antibody [EPR21706-126] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-7 antibody [EPR21706-126] - BSA and Azide free (Detector) (AB271310)

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Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242630 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL-15. The recommended pair for sandwich ELISA is:
Capture: ab242630, Human IL-15 Capture Antibody (unconjugated)
Detector: ab242875, Human IL-15 Detector Antibody (unconjugated)
The reference range value is 7.8 - 500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8178155, PubMed : 9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed : 9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed : 10233906, PubMed : 23104097, PubMed : 8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed : 7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
See full target information IL15

대체 명칭 보기

Interleukin-15, IL-15, IL15
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 9:e97567 PubMed24824861

2014

Development of rabbit monoclonal antibodies for detection of alpha-dystroglycan in normal and dystrophic tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa J Fortunato,Charlotte E Ball,Katrin Hollinger,Niraj B Patel,Jill N Modi,Vedika Rajasekaran,Dan J Nonneman,Jason W Ross,Eileen J Kennedy,Joshua T Selsby,Aaron M Beedle
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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