- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-IL-17A antibody [4K5F6] (AB189377)
Intracellular flow cytometric analysis of IL-17A in Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, labeling IL-17A with ab189377 at 0.1 ug/106 cells (red) Green represents isotype control antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [4K5F6] (AB189377)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labelling IL-17A with ab189377 at 10 μg/ml.
- WB
Supplier Data
Western blot - Anti-IL-17A antibody [4K5F6] (AB189377)
All lanes:
Western blot - Anti-IL-17A antibody [4K5F6] (ab189377) at 0.2 µg/mL
Lane 1:
Human full-length recombinant IL-17A protein at 0.1 µg/mL
Lane 2:
Mouse full-length recombinant IL-17A protein at 0.1 µg/mL
Lane 3:
Rat full-length recombinant IL-17A protein at 0.1 µg/mL
Predicted band size: 18 kDa
false
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