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AB240376

Anti-IL-18 antibody [EPR19956] - BSA and Azide free

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal IL-18 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.

대체 명칭 보기

Igif, Il18, Interleukin-18, IL-18, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

3 이미지
Immunoprecipitation - Anti-IL-18 antibody [EPR19956] - BSA and Azide free (AB240376)
  • IP

Supplier Data

Immunoprecipitation - Anti-IL-18 antibody [EPR19956] - BSA and Azide free (AB240376)

IL-18 was immunoprecipitated from 0.35 mg of mouse spleen lysate with ab207323 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse spleen lysate 10μg (Input).

Lane 2 : ab207323 IP in mouse spleen lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207323 in mouse spleen lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207323).

All lanes:

Immunoprecipitation - Anti-IL-18 antibody [EPR19956] (<a href='/ko/products/primary-antibodies/il-18-antibody-epr19956-ab207323'>ab207323</a>)

Predicted band size: 22 kDa

false

Western blot - Anti-IL-18 antibody [EPR19956] - BSA and Azide free (AB240376)
  • WB

Supplier Data

Western blot - Anti-IL-18 antibody [EPR19956] - BSA and Azide free (AB240376)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207323).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-18 antibody [EPR19956] (<a href='/ko/products/primary-antibodies/il-18-antibody-epr19956-ab207323'>ab207323</a>) at 1/1000 dilution

All lanes:

Mouse IL-18 active protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa,21 kDa

Observed band size: 18 kDa

false

Exposure time: 1s

Western blot - Anti-IL-18 antibody [EPR19956] - BSA and Azide free (AB240376)
  • WB

Supplier Data

Western blot - Anti-IL-18 antibody [EPR19956] - BSA and Azide free (AB240376)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207323).

Blocking/Dilution buffer : 5% NFDM/TBST.

The protein expression level can refer to reference PMID 10051628.

All lanes:

Western blot - Anti-IL-18 antibody [EPR19956] (<a href='/ko/products/primary-antibodies/il-18-antibody-epr19956-ab207323'>ab207323</a>) at 1/1000 dilution

All lanes:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Exposure time: 3min

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19956

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB223293

Anti-IL-18 antibody [EPR22249-212]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (AB223293)

  • 0
  • 0
Primary Antibodies

AB243091

Anti-IL-18 antibody [EPR19954-188]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (AB243091)

  • 0
  • 0
Primary Antibodies

AB222926

Anti-IL-18 antibody [EPR19954] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (AB222926)

  • 0
  • 0
Primary Antibodies

AB243295

Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (AB243295)

  • 3
  • 1
Primary Antibodies

AB242022

Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (AB242022)

  • 0
  • 0
Primary Antibodies

AB207324

Anti-IL-18 antibody [EPR19954]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] (AB207324)

  • 0
  • 0

보완 제품

Assay Kits

AB215539

Human IL-18 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-18 ELISA Kit (AB215539)

  • 5
  • 1
Primary Antibodies

AB263899

Anti-NLRP3 antibody [EPR23094-1]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-NLRP3 antibody [EPR23094-1] (AB263899)

  • 4
  • 2
Primary Antibodies

AB179515

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

  • 3
  • 3
Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (AB283818)

  • 0
  • 0
Primary Antibodies

AB219800

Anti-GSDMD antibody [EPR20859]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-GSDMD antibody [EPR20859] (AB219800)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

ab240376 is the carrier-free version of ab207323.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 26638072, PubMed : 26638073). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (By similarity). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (By similarity). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 30392956).
See full target information Il18
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 15:926 PubMed39715736

2024

Sphk1/S1P pathway promotes blood-brain barrier breakdown after intracerebral hemorrhage through inducing Nlrp3-mediated endothelial cell pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhao Feng,Yuan An,Qi Qin,Iat-Hang Fong,Kaiyuan Zhang,Fang Wang,Dengpan Song,Mengyuan Li,Min Yu,Chi-Tai Yeh,Junlei Chang,Fuyou Guo

Signal transduction and targeted therapy 8:358 PubMed37735155

2023

Tauopathy promotes spinal cord-dependent production of toxic amyloid-beta in transgenic monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuchi Tu,Sen Yan,Bofeng Han,Caijuan Li,Weien Liang,Yingqi Lin,Yongyan Ding,Huiyi Wei,Lu Wang,Hao Xu,Jianmeng Ye,Bang Li,Shihua Li,Xiao-Jiang Li
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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