AB167159

Anti-IL-3 antibody [EPR7964]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal IL-3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (AB167159)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (AB167159)

Immunocytochemical analysis of Jurkat cells labeling IL3 with ab167159 at a 1/100 dilution.

Unknown

Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

All lanes:

Western blot - Anti-IL-3 antibody [EPR7964] (ab167159) at 1/1000 dilution

All lanes:

Human IL3 recombinant protein (10 ng)

Predicted band size: 17 kDa

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Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

Blocking/diluting Buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-IL-3 antibody [EPR7964] (ab167159) at 1.23 µg/mL

All lanes:

Mouse IL-3 recombinant protein at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7964

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

WB is only recommended to detect recombinant protein, not endogenous IL-3.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 2556442). Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated (PubMed : 10779277, PubMed : 32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed : 23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed : 12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed : 29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed : 27862234).

See full target information IL3

대체 명칭 보기

Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405364 PubMed39225429

2024

Nanotherapeutic Approaches of Interleukin-3 to Clear the α-Synuclein Pathology in Mouse Models of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlong Zhang,Jian Ren,Liuyan Ding,Shaohui Zheng,Runfang Ma,Mengran Zhang,Yan Liu,Ruijing Liang,Yunlong Zhang

Oncology letters 17:1523-1530 PubMed30675208

2019

Evidence for a role of geranylgeranylation in renal angiomyolipoma and renal epithelioid angiomyolipoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Zhao,Jun Yuan,Qi Cheng,Ya-Ling Qi,Ke Lu,Shan-Shan Lai,Qian Sun,Yue Zhao,Lei Fang,Mei-Ling Jin,De-Cai Yu,Yu-Dong Qiu,Chao-Jun Li,Jun Chen,Bin Xue

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-3 antibody [EPR7964]

Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (AB167159)

Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Assay Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise