JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB53695

Anti-IL-3RB (phospho Y593) antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal IL-3RB phospho Y593 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CSF2RB pY593 aa 550-650.
2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-3RB (phospho Y593) antibody (AB53695)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-3RB (phospho Y593) antibody (AB53695)

ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Western blot - Anti-IL-3RB (phospho Y593) antibody (AB53695)
  • WB

Unknown

Western blot - Anti-IL-3RB (phospho Y593) antibody (AB53695)

All lanes:

Western blot - Anti-IL-3RB (phospho Y593) antibody (ab53695)

Lane 1:

HepG2 cells treated with EGF (200ng/ml, 15 minutes)

Lane 2:

HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide

Predicted band size: 97 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CSF2RB pY593 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

P32927

Specificity

ab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA (PubMed : 1495999). In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK-STAT pathway (PubMed : 9516124).
See full target information CSF2RB pY593

대체 명칭 보기

CD131, IL3RB, IL5RB, CSF2RB, Cytokine receptor common subunit beta, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Breast cancer research and treatment 175:353-368 PubMed30830488

2019

ERα upregulates the expression of long non-coding RNA LINC00472 which suppresses the phosphorylation of NF-κB in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanwei Wang,Dionyssios Katsaros,Nicoletta Biglia,Yi Shen,Lenora Loo,Xiao Yu,Hongyan Lin,Yuanyuan Fu,Wen-Ming Chu,Peiwen Fei,Yan Ni,Wei Jia,Xiaobei Deng,Biyun Qian,Herbert Yu
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com