AB203398

Anti-IL-4R antibody

Be the first to review this product! 리뷰 제출

(14 제품이 사용된 논문 )

Rabbit Polyclonal IL-4R antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human IL4R aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA

4 이미지

Immunocytochemistry/ Immunofluorescence - Anti-IL-4R antibody (AB203398)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IL-4R antibody (AB203398)

Immunocytochemistry/ Immunofluorescence analys of A431 cells labeling IL-4R with ab203398 1/100 for 90 minutes at 37°C. Cells were fixed with 4% Paraformaldehyde, Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min. Secondary antibody was Goat Anti-Rabbit IgG antibody with FITC conjugation at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.

Flow Cytometry - Anti-IL-4R antibody (AB203398)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-4R antibody (AB203398)

Flow Cytometry analysis of Raji cells labeling IL-4R with ab203398. Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining with ab203398 was performed at 1/100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used at a 1/200 dilution for 40 min at room temperature. Primary antibody staining (green) is compared to compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4R antibody (AB203398)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4R antibody (AB203398)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling IL4R with ab203398 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4R antibody (AB203398)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for IL4R using ab203398 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated at 1/200 dilution.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human IL4R aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P24394

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

See full target information IL4R

style

left-column

제품이 사용된 논문 (14)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine reports 32: PubMed41041835

2025

Regulatory role of POSTN in keloid pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Jiang,Fan Zhuo,Xiahong Li,Kaoyuan Zhang,Jiaxu Gu,Jingwen Wu,Weilong Zhong,Yanfen Zou,Bo Yu,Cong Huang

Open veterinary journal 15:3558-3570 PubMed41036017

2025

Host-parasite interaction in latent cerebral toxoplasmosis: The role of cytokine response in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Tuğçe Anteplioğlu,Merve Bişkin Türkmen,Erva Eser,Damla Okatan,Oğuz Kul

Chinese medicine 20:34 PubMed40069808

2025

Chlorogenic acid attenuates pyrrolizidine alkaloid-induced liver injury through modulation of the SIRT1/FXR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Qiongwen Xue,Aizhen Xiong,Yilin Chen,Xunjiang Wang,Xing Yan,Deqing Ruan,Yumeng Zhang,Zhengtao Wang,Lili Ding,Li Yang

Frontiers in immunology 15:1450887 PubMed39867900

2025

NLRP3 deficiency aggravated DNFB-induced chronic itch by enhancing type 2 immunity IL-4/TSLP-TRPA1 axis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Ting Huang,Zuo-Ming Chen,Zhe Peng,Yu Wang,Fan Yang,Yang Tang,Zi Li,Li Wan

The Journal of dermatology 51:e321-e323 PubMed38605435

2024

Expression of phosphorylated-Janus kinase 1 and IL-4Rα by dermal basophils and epidermal keratinocytes in atopic dermatitis-associated prurigo nodules: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Riichiro Sugiura,Takashi Hashimoto,Satoshi Okuno,Takahiro Satoh

Journal of the European Academy of Dermatology and Venereology : JEADV 38:895-903 PubMed38084871

2023

Mechanism underlying pruritus in recessive dystrophic epidermolysis bullosa: Role of interleukin-31 from mast cells and macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Gyun Lee,Song-Ee Kim,In-Hye Jeong,Sang Eun Lee

Cells 12: PubMed37296643

2023

In Situ Identification of Both IL-4 and IL-10 Cytokine-Receptor Interactions during Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztina Nikovics,Anne-Laure Favier,Mathilde Rocher,Céline Mayinga,Johanna Gomez,Frédérique Dufour-Gaume,Diane Riccobono

Scientific reports 13:6964 PubMed37117331

2023

Prognosis value and positive association of Rab1A/IL4Rα aberrant expression in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Li,Zhengwu Cheng,Bin Jiang,Xinyu Shao,Menglin Xu

Translational stroke research 14:250-262 PubMed35867328

2022

Three Days Delayed Recanalization Improved Neurological Function in pMCAO Rats by Increasing M2 Microglia-Possible Involvement of the IL-4R/STAT6/PPARγ Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqing Kang,Marcin Gamdzyk,Yujie Luo,Hong Tang,Lei Huang,Cameron Lenahan,Desislava Doycheva,Dujuan Li,Jiping Tang,Sheng Tan,John H Zhang

The Journal of experimental medicine 219: PubMed35587822

2022

Interleukin-4 receptor signaling modulates neuronal network activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Hanuscheck,Carine Thalman,Micaela Domingues,Samantha Schmaul,Muthuraman Muthuraman,Florian Hetsch,Manuela Ecker,Heiko Endle,Mohammadsaleh Oshaghi,Gianvito Martino,Tanja Kuhlmann,Katarzyna Bozek,Tim van Beers,Stefan Bittner,Jakob von Engelhardt,Johannes Vogt,Christina Francisca Vogelaar,Frauke Zipp

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-4R antibody

Immunocytochemistry/ Immunofluorescence - Anti-IL-4R antibody (AB203398)

Flow Cytometry - Anti-IL-4R antibody (AB203398)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4R antibody (AB203398)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4R antibody (AB203398)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (14)

Product promise