![Sandwich ELISA - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR20150-201] - BSA and Azide free (Capture) (AB244608)](https://content.abcam.com/products/images/il1-receptor-i-il-1r-1-antibody-epr20150-201-bsa-and-azide-free-capture-ab244608--sandwich-elisa-img52435.jpg/_jcr_content/renditions/webp-768.webp)
- sELISA
Supplier Data
Sandwich ELISA - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR20150-201] - BSA and Azide free (Capture) (AB244608)
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab260072).
보완 제품
Primary Antibodies
AB284960
Anti-CD27 antibody [EPR24292-105] - BSA and Azide free (Capture)
RabMAb|Recombinant
![Sandwich ELISA - Anti-CD27 antibody [EPR24292-105] - BSA and Azide free (Capture) (AB284960)](https://content.abcam.com/products/images/cd27-antibody-epr24292-105-bsa-and-azide-free-capture-ab284960--sandwich-elisa-img164459.jpg)
- 0
- 0
Primary Antibodies
AB278014
Anti-RANK antibody [EPR23308-30] - BSA and Azide free (Detector)
RabMAb|Recombinant
![Sandwich ELISA - Anti-RANK antibody [EPR23308-30] - BSA and Azide free (Detector) (AB278014)](https://content.abcam.com/products/images/rank-antibody-epr23308-30-bsa-and-azide-free-detector-ab278014--sandwich-elisa-img136742.jpg){kind=icon}
- 0
- 0
Primary Antibodies
AB314571
Anti-LTBR antibody [EPR26646-39] - BSA and Azide free (Detector)
RabMAb|Recombinant
![Sandwich ELISA - Anti-LTBR antibody [EPR26646-39] - BSA and Azide free (Detector) (AB314571)](https://content.abcam.com/products/images/ltbr-antibody-epr26646-39-bsa-and-azide-free-detector-ab314571--sandwich-elisa-img422514.jpg)
- 0
- 0
Primary Antibodies
AB244966
Anti-CD137 antibody [EPR20238-121] - BSA and Azide free (Detector)
RabMAb|Recombinant
![Sandwich ELISA - Anti-CD137 antibody [EPR20238-121] - BSA and Azide free (Detector) (AB244966)](https://content.abcam.com/products/images/cd137-antibody-epr20238-121-bsa-and-azide-free-detector-ab244966--sandwich-elisa-img51910.jpg)
- 0
- 0
Reactivity 정보
제품 세부 정보
ab244608 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL1R1. The recommended pair for sandwich ELISA is:
Capture: ab244608, Human IL1R1 Capture Antibody (unconjugated)
Detector: ab244859, Human IL1R1 Detector Antibody (unconjugated)
The reference range value is 187.5 - 12000 pg/ml.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IL-1R1 is a critical player in the initiation of immune responses serving as a component of the IL-1 signaling complex. When IL-1 binds to IL-1R1 it recruits the IL-1 receptor accessory protein (IL-1RAcP) to form a signaling complex that triggers downstream pathways. This interaction initiates a signaling cascade that activates nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) promoting the expression of inflammatory genes and mediating immune and inflammatory responses.
Pathways
IL-1R1 is central to the inflammatory response and immune signaling pathways. The IL-1 receptor complex facilitates the activation of NF-κB which plays a role in the body's response to stress cytokines and pathogens. The receptor acts in close relation with other proteins like IL-1RAcP and IRAKs (Interleukin-1 receptor-associated kinases) in these pathways creating a robust immune signaling network that drives the inflammatory response.
제품 프로토콜
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타겟 정보
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com