AB324945

Anti-IL22 RA2/IL-22BP antibody [EPR27285-7]

RabMAb
Recombinant
20ul selling size
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Rabbit Recombinant Monoclonal IL22 RA2/IL-22BP antibody. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.

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Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte, Left) / DCs (mouse dendritic cell sarcoma cell, Right) cells labelling IL22 RA2/IL-22BP with ab324945 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Low expression : WEHI-231 (PMID : 23653115).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse bone marrow derived Dendritic cells induced by 20ng/ml GM-CSF for 13 days and 50ng/ml IL4 for 7 days ((Lower left and right) Mouse bone marrow (Upper left and right)) cells labelling IL22 RA2/IL-22BP with ab324945 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti-CD11c conjugated to BV421 and anti-MHCII conjugated to APC/CY7, then fixed with 2% PFA for 10 min followed by intracellular staining.
Gated on viable CD11c positive cells.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27285-7

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.

See full target information Il22ra2

대체 명칭 보기

Interleukin-22 receptor subunit alpha-2, IL-22 receptor subunit alpha-2, IL-22R-alpha-2, IL-22RA2, Interleukin-22-binding protein, ZcytoR16, CRF2-S1, IL-22BP, IL22BP

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL22 RA2/IL-22BP antibody [EPR27285-7]

Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)

Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise