Anti-ILT-4 antibody [EPR29046-643] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILT-4 antibody [EPR29046-643] - BSA and Azide free (AB327064)

This data was developed using ab327056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) : HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human LILRB2 expression vector containing a his tag. (B) : HEK-293T cells transfected with a human LILRB1 expression vector containing a his tag. (C) : HEK-293T cells transfected with a human LILRA1 expression vector containing a his tag. (D) : HEK-293T cells transfected with a human LILRA3 expression vector containing a his tag. (E) : HEK-293T cells transfected with a human LILRA2 expression vector containing a his tag. (F) : HEK-293T cells transfected with a human LILRA6 expression vector containing a his tag. (G) : HEK-293T cells transfected with a human LILRB3 expression vector containing a his tag. (H) : HEK-293T cells transfected with a human LILRB5 expression vector containing a his tag. (I) : HEK-293T cells transfected with empty vector containing a his tag. tissue labeling ILT-4 with ab327056 at 1/2000 (0.251 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a human LILRB2 expression vector containing a his tag, no staining on (B) HEK-293T cells transfected with a human LILRB1 expression vector containing a his tag, (C) HEK-293T cells transfected with a human LILRA1 expression vector containing a his tag, (D) HEK-293T cells transfected with a human LILRA3 expression vector containing a his tag, (E) HEK-293T cells transfected with a human LILRA2 expression vector containing a his tag, (F) HEK-293T cells transfected with a human LILRA6 expression vector containing a his tag, (G) HEK-293T cells transfected with a human LILRB3 expression vector containing a his tag, (H) HEK-293T cells transfected with a human LILRB5 expression vector containing a his tag, and (I) HEK-293T cells transfected with empty vector containing a his tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILT-4 antibody [EPR29046-643] - BSA and Azide free (AB327064)

This data was developed using ab327056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling ILT-4 with ab327056 at 1/100 (5.02 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human colon cancer.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILT-4 antibody [EPR29046-643] - BSA and Azide free (AB327064)

This data was developed using ab327056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ILT-4 with ab327056 at 1/100 (5.02 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : positive staining on immune cells of human skeletal muscle.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILT-4 antibody [EPR29046-643] - BSA and Azide free (AB327064)

This data was developed using ab327056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma tissue labeling ILT-4 with ab327056 at 1/100 (5.02 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human non-Hodgkin's lymphoma.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILT-4 antibody [EPR29046-643] - BSA and Azide free (AB327064)

This data was developed using ab327056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling ILT-4 with ab327056 at 1/100 (5.02 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-ILT-4 antibody [EPR29046-643] - BSA and Azide free (AB327064)

This data was developed using ab327056, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : 293T, Hela.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ILT-4 antibody [EPR29046-643] (<a href='/ko/products/primary-antibodies/ilt-4-antibody-epr29046-643-ab327056'>ab327056</a>) at 1/1000 dilution

Lane 1:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 80 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 80 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 180s