AB248833

Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IMPDH1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

IMPD1, IMPDH1, Inosine-5'-monophosphate dehydrogenase 1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPDH-I, IMPD2, IMPDH2, Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, Inosine-5'-monophosphate dehydrogenase type II, IMP dehydrogenase II, IMPDH-II

2 이미지

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Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] - BSA and Azide free (AB248833)

This data was developed using ab137120, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] (<a href='/ko/products/primary-antibodies/impdh2-impdh1-antibody-epr9678b-ab137120'>ab137120</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

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Lab

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] - BSA and Azide free (AB248833)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] (<a href='/ko/products/primary-antibodies/impdh2-impdh1-antibody-epr9678b-ab137120'>ab137120</a>) at 1/1000 dilution

Lane 1:

DDK tagged human full length IMPDH1 recombinant protein (P20839-3) at 10 ng

Lane 2:

DDK tagged human full length IMPDH2 recombinant protein (P12268) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,55 kDa

false

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9678(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 92% homology with IPMDH2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab248833 is the carrier-free version of ab137120.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The proteins IMPDH2 and IMPDH1 also known as inosine-5'-monophosphate dehydrogenase 2 and 1 are enzymes involved in purine metabolism. IMPDH1 has a mass of approximately 55 kDa while IMPDH2 measures around 56 kDa. Both proteins catalyze the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) an essential step in the de novo synthesis of guanine nucleotides. They are expressed in various tissues with highest levels found in blood and immune system cells reflecting their important role in cell proliferation.

Biological function summary

These enzymes perform critical functions by facilitating the synthesis of guanine nucleotides which are building blocks of DNA and RNA. IMPDH1 and IMPDH2 do not function within large multimeric complexes but exhibit regulatory roles over cellular proliferation and differentiation. The balance between these enzymes impacts the overall guanine nucleotide pool affecting cellular processes such as growth and division.

Pathways

These enzymes link to nucleotide biosynthesis pathways particularly the guanine nucleotide synthesis pathway. This pathway integrates with the purine metabolism network. IMPDH proteins interact with key enzymes like GMP synthase which directly follows IMPDHs in nucleotide synthesis. Their activity regulates cell proliferative capacity making them integral to processes like DNA replication and repair.

The proteins play significant roles in conditions like cancer and retinitis pigmentosa. IMPDH2 is often overexpressed in rapidly proliferating cancer cells linking it to tumor growth and progression. Meanwhile mutations in IMPDH1 have associations with retinitis pigmentosa a genetic disorder causing retinal degeneration. These enzymes’ dysfunction relates to proteins they interact with in these diseases such as DNA repair proteins in cancer and photoreceptor proteins in retinal disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

See full target information IMPDH1

Additional targets

IMPDH2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] - BSA and Azide free

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] - BSA and Azide free (AB248833)

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] - BSA and Azide free (AB248833)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

Product promise