Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29]

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519)

Indirect ELISA analysis of ab322519 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin / HA Protein.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (ab322519) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with a Influenza A Virus H1N1 (A/California/04/2009) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with a Influenza A Virus H5N1 (strain A/Vietnam/1203/2004 H5N1) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/10000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 4s