Anti-Influenza A Virus PB2 protein antibody [EPR28249-46]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (AB312856)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A Virus PB2 protein with ab312856 at 1/1000 (0.513 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in 293T cells transfected with a Influenza A Virus PB2 protein expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 (0.38ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (AB312856)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Influenza A Virus PB2 protein with ab312856 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human kidney. The section was incubated with ab312856 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (AB312856)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Influenza A Virus PB2 protein with ab312856 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse kidney.The section was incubated with ab312856 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (AB312856)

Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T c tissue labeling Influenza A Virus PB2 protein with ab312856 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on (A) HEK-293T transfected with a Influenza A Virus PB2 protein expression vector and no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab312856 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (AB312856)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / HEK-293T (human embryonic kidney epithelial cells) transfected with a Influenza A Virus PB2 protein expression vector containing a myc-his tag (Middle) / HEK-293T cells transfected with empty vector containing a myc-his tag (Right) cells labelling Influenza A Virus PB2 protein with ab312856 at 1/500 dilution (0.1 ug)/ Middle and Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• I-ELISA

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Indirect ELISA - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (AB312856)

Indirect ELISA analysis of ab312856 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Influenza A Virus PB2 protein.

Antigen concentration : 1000 ng/ml

• WB

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Western blot - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (AB312856)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 7 seconds

All lanes:

Western blot - Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] (ab312856) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

HEK-293 transfected with a Influenza A Virus PB2 protein expression vector containi a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 86 kDa

false

Exposure time: 7s