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AB244387

Anti-ING2 antibody

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Rabbit Polyclonal ING2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ING2 aa 1-100.

대체 명칭 보기

ING1L, ING2, Inhibitor of growth protein 2, Inhibitor of growth 1-like protein, p32, p33ING2, ING1Lp

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ING2 antibody (AB244387)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ING2 antibody (AB244387)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ING2 (green) using ab244387 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ING2 antibody (AB244387)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ING2 antibody (AB244387)

Paraffin-embedded human gallbladder tissue stained for ING2 using ab244387 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-ING2 antibody (AB244387)
  • WB

Unknown

Western blot - Anti-ING2 antibody (AB244387)

All lanes:

Western blot - Anti-ING2 antibody (ab244387) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

ING2 overexpression HEK-293T cell lysate

Predicted band size: 33 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ING2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9H160

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대체품

Primary Antibodies

AB174305

Anti-ING2 antibody [EPR4597-5]

RabMAb|Recombinant

Immunoprecipitation - Anti-ING2 antibody [EPR4597-5] (AB174305)

  • 0
  • 0

보완 제품

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB129195

Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (AB129195)

  • 4
  • 5
Primary Antibodies

AB246550

Anti-p53 antibody [EPR20416-120]

RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-120] (AB246550)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ING2 also known as Inhibitor of Growth Protein 2 is a nuclear protein with a molecular weight of approximately 33 kDa. It expresses highly in tissues such as the prostate placenta and lung. Nuclear localization signals and PHD-finger motifs characterize its structure allowing it to bind to chromatin and influence gene expression. As a member of the ING family ING2 plays a role in regulating various cellular processes through its presence in different cellular compartments.
Biological function summary

ING2 functions as a tumor suppressor by controlling the transcription of genes involved in cell cycle regulation and apoptosis. It interacts with histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes contributing to chromatin remodeling. As part of the Sin3A-HDAC complex ING2 has the ability to repress transcription influencing the expression of genes important for maintaining cellular homeostasis and preventing oncogenesis.

Pathways

The ING2 protein actively participates in the p53 signaling pathway as well as the MAPK signaling pathway. Its interaction with p53 leads to the enhancement of p53-mediated transcriptional activation which promotes cell cycle arrest and apoptosis. ING2 also interacts with proteins like TP53 and EP300 modulating the cellular responses to stress signals and contributing to cell survival and repair mechanisms.

The involvement of ING2 has been linked to cancer and neurodegenerative disorders. In cancer downregulation or mutation of ING2 disrupts the p53 pathway leading to impaired tumor suppression. ING2's interaction with TP53 and its tumor suppressor role highlights its importance in cancer biology. Furthermore in neurodegenerative disorders ING2's dysregulation can influence neuronal survival pathways potentially affecting proteins like p53 and EP300 therefore altering neuronal function and contributing to disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
See full target information ING2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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