AB113425

Anti-ING4 antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal ING4 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ING4 aa 1-200.

대체 명칭 보기

My036, ING4, Inhibitor of growth protein 4, p29ING4

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ING4 antibody (AB113425)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ING4 antibody (AB113425)

ab113425 at 1/100 dilution staining ING4 in Formalin-fixed, Paraffin-embedded Human fetal brain tissue by Immunohistochemistry. Note nuclear staining.

Unknown

Western blot - Anti-ING4 antibody (AB113425)

All lanes:

Western blot - Anti-ING4 antibody (ab113425) at 1/500 dilution

Lane 1:

Human fetal liver lysate

Lane 2:

Human fetal kidney lysate

Lane 3:

Mouse kidney lysate

Predicted band size: 29 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ING4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UNL4

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Lyophilized

Reconstitution

Reconstitute in 200 µL of water

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab113425 is purified by a protein affinity column.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ING4 also known as Inhibitor of Growth Family Member 4 is a nuclear protein with a molecular mass of approximately 29 kDa. This protein mainly localizes to the nucleus and is expressed in various tissues including the brain and liver. ING4 contains a plant homeodomain (PHD) finger motif which suggests its involvement in chromatin-mediated transcriptional regulation. As a regulator of gene expression ING4 influences cell growth and apoptosis by interacting with histones to affect chromatin structure and function.

Biological function summary

The ING4 protein plays a significant role in tumor suppression. It forms part of a larger protein complex by associating with components like the nucleosome remodeling and histone deacetylase (NuRD) complex. By binding to specific histone modifications ING4 influences transcriptional regulation of several genes that control cell cycle and apoptosis. This activity helps maintain genomic stability and prevent cellular transformation establishing ING4 as a guardian against oncogenic processes.

Pathways

ING4 integrates into cellular machinery that governs the NF-κB signaling pathway and the p53 pathway. These pathways are critical for controlling inflammation and cellular stress responses. Within the NF-κB pathway ING4 interacts with proteins such as IκBα modulating inflammatory cytokine production. In the context of the p53 pathway ING4 acts together with p53 enhancing its tumor suppressor functions through transcriptional activation of p53-responsive genes.

ING4 has a notable association with cancer particularly glioblastoma and breast cancer. Reduced expression or alterations in ING4 have been linked to these malignancies where it functions to suppress the aggressive characteristics of tumor cells. ING4 also connects with proteins like HIF-1α in pathways that regulate cellular responses to hypoxia further implicating its role in tumor biology. Understanding ING4's interactions and effects offers potential for developing therapeutic strategies targeting cancer progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), and have reduced activity toward histone H4 (PubMed : 16387653). Through chromatin acetylation it may function in DNA replication (PubMed : 16387653). May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation (PubMed : 15251430, PubMed : 15528276). Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA (PubMed : 15029197). May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC (PubMed : 15029197). Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1) (PubMed : 15897452). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity).

See full target information ING4

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

CytoJournal 21:77 PubMed39917007

2025

Investigating the role of exosomal microRNA-5703 in modulating tumor-associated endothelial cells in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Wen,Rancen Tao,Yuyu Liu,Zhenfa Zhang

Oncology letters 21:67 PubMed33365078

2020

Lentiviral vector with a radiation-inducible promoter, carrying the ING4 gene, mediates radiosensitization controlled by radiotherapy in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ma,Rui Guo,Xi Wang,Wen-Tong Shen,Min Zhu,Ye-Ning Jin,Hao-Ping Xu

Oncology letters 18:4621-4628 PubMed31611970

2019

miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangqin Tang,Yanjun Ding,Xiaoqing Wang,Xiuzhen Wang,Lin Zhao,Hongmei Bi

Journal of cellular biochemistry 120:6709-6717 PubMed30390334

2018

Inhibitor of growth 4 inhibits cell proliferation, migration, and induces apoptosis of renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiping Sun,Liyi Xie,Jing Lv,Wenjing Zhang,Jia Lv,Yu Liang,Yingzhou Geng,Xudong Li

Oncology letters 14:3663-3668 PubMed28927128

2017

Expression of ING4 is negatively correlated with cellular proliferation and microvessel density in human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Shao,Enzhong Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ING4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ING4 antibody (AB113425)

Western blot - Anti-ING4 antibody (AB113425)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Reconstitution

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise