AB235905

Anti-Integrin alpha 6 antibody

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(9 제품이 사용된 논문 )

Rabbit Polyclonal Integrin alpha 6 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGA6 aa 600-900.

대체 명칭 보기

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody (AB235905)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody (AB235905)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells laneling Integrin alpha 6 using ab235905 at 1/200 dilution in ICC/IF. Cells were blocked in 10% normal goat serum and incubated with primary antibody overnight at 4°C. Secondary antibody was Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (H+L).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITGA6 aa 600-900. The exact immunogen used to generate this antibody is proprietary information.

P23229

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin's structural stability.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6 : ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGA6

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Breast cancer research : BCR 26:187 PubMed39707454

2024

Cilengitide sensitivity is predicted by overall integrin expression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nomeda Girnius,Aylin Z Henstridge,Benjamin Marks,Jeffrey K Yu,G Kenneth Gray,Chris Sander,Ioannis K Zervantonakis,Augustin Luna

Frontiers in immunology 15:1394530 PubMed38881903

2024

Poly-l-lactic acid microspheres delay aging of epidermal stem cells in rat skin.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxian Dong,Youliang Zhang,Hao Yu,Lingcong Zhou,Yaan Zhang,Haibin Wang,Zhicheng Hu,Shengkang Luo

Stem cell research & therapy 14:51 PubMed36959609

2023

Single-stage transplantation combined with epidermal stem cells promotes the survival of tissue-engineered skin by inducing early angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Wang,Hailin Xu,Hao Yang,Yi Zhang,Xiaoyan Wang,Peng Wang,Zhongye Xu,Dongming Lv,Yanchao Rong,Yunxian Dong,Bing Tang,Zhicheng Hu,Wuguo Deng,Jiayuan Zhu

Cell reports 41:111580 PubMed36323248

2022

Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa E L Romano,Wen Yih Aw,Kathryn M Hixson,Tatiana V Novoselova,Tammy M Havener,Stefanie Howell,Bonnie Taylor-Blake,Charlotte L Hall,Lei Xing,Josh Beri,Suran Nethisinghe,Laura Perna,Abubakar Hatimy,Ginevra Chioccioli Altadonna,Lee M Graves,Laura E Herring,Anthony J Hickey,Konstantinos Thalassinos,J Paul Chapple,Justin M Wolter

Stem cell research & therapy 13:346 PubMed35883101

2022

Melatonin in cryopreservation media improves transplantation efficiency of frozen-thawed spermatogonial stem cells into testes of azoospermic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shokoofeh Kazemzadeh,Shahram Mohammadpour,Soheila Madadi,Azar Babakhani,Maryam Shabani,Maryam Khanehzad

Upsala journal of medical sciences 126: PubMed34471485

2021

LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chuancheng Sun,Bing Xu,Liang Wang,Yilin Su

Cancer cell international 20:574 PubMed33317527

2020

Exosomal miR-126 blocks the development of non-small cell lung cancer through the inhibition of ITGA6.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjun Li,Qianqian Wang,Xiaofei Zhang,Ningning Yan,Xingya Li

Stem cell research & therapy 11:344 PubMed32771044

2020

Rat epidermal stem cells promote the angiogenesis of full-thickness wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Shaobin Huang,Zhicheng Hu,Peng Wang,Yi Zhang,Xiaoling Cao,Yunxian Dong,Pu Cheng,Hailin Xu,Wenkai Zhu,Bing Tang,Jiayuan Zhu

International journal of molecular medicine 43:517-524 PubMed30365062

2018

miR-186 reverses cisplatin resistance and inhibits the formation of the glioblastoma-initiating cell phenotype by degrading Yin Yang 1 in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Jie Song,Feng Guo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody (AB235905)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (9)

Product promise