AB227702

Anti-Integrin beta 3 antibody [SP324]

20ul selling size
RabMAb
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Rabbit Monoclonal Integrin beta 3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications.

5 이미지

Multiplex immunohistochemistry - Anti-Integrin beta 3 antibody [SP324] (AB227702)

mIHC

Lab

Multiplex immunohistochemistry - Anti-Integrin beta 3 antibody [SP324] (AB227702)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining with ab327706 at a 1 : 100 (4.87 μg/ml) dilution, ab227702 anti-Integrin beta 3 used at 1 : 100 (0.40 μg/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 μg/ml) dilution.

Panel A : merged staining of anti-CLEC-2 (green; Opal™520), anti-Integrin beta 3 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on human spleen.
Panel B : anti-CLEC-2 staining platelets in human spleen.
Panel C : anti-Integrin beta 3 staining platelets in human spleen.
Panel D : anti-CD3 staining T lymphocytes in human spleen.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab327706, ab227702 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [SP324] (AB227702)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [SP324] (AB227702)

Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [SP324] (AB227702)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Integrin beta 3 antibody [SP324] (AB227702)

All lanes:

Western blot - Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/100 dilution

All lanes:

HUVEC (human umbilical vein endothelial cell line) cell lysate

Predicted band size: 87 kDa

false

Lab

Western blot - Anti-Integrin beta 3 antibody [SP324] (AB227702)

LnCaP, HeLa and PC-3 are low expression controls. This product doesn't react with Ms

All lanes:

Western blot - Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/500 dilution

Lane 1:

U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEL (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg

Lane 3:

LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 5:

HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg

Lane 6:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 7:

C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

Lane 8:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Lane 9:

Mouse brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 110 kDa

false

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP324

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "Primary antibody incubation for 1 hour at room temperature.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.Primary antibody incubation for 10 minutes at room temperature." }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "Primary antibody incubation for 1 hour at room temperature.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS by protein A/G.

보관 버퍼

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 3 also known as ITGB3 beta 3 integrin or CD41/CD61 is a cell surface receptor that plays a role in cell adhesion and signal transduction. It is a member of the integrin family characterized by a heterodimer structure formed with an alpha subunit. This integrin has a molecular mass of approximately 88 kDa. Integrin beta 3 is expressed on platelets megakaryocytes and several other cell types such as endothelial cells and certain tumor cells.

Biological function summary

Integrins serve as the mechanical link between the extracellular matrix and the cytoskeleton. Beta 3 integrin forms a complex with different alpha subunits most notably alpha IIb to function as a receptor for fibrinogen fibronectin and vitronectin. This receptor complex is essential for platelet aggregation and normal hemostasis. Dysregulation can lead to altered cellular responses impacting processes like immune response and tumor progression.

Pathways

Integrin beta 3 participates in the signaling of outside-in and inside-out pathways that are fundamental in cellular dynamics. Of particular significance are the integrin-mediated signaling pathways and the TGF-beta signaling pathway which influence cellular proliferation and differentiation. Integrin beta 3 interacts with proteins such as talin and kindlin which are vital for activation and clustering of integrins during cell signaling processes.

Altered expression or function of integrin beta 3 is associated with bleeding disorders like Glanzmann's thrombasthenia where platelet aggregation is impaired. Additionally it plays a role in the progression of certain cancers due to its involvement in cell migration and invasion. The interaction of integrin beta 3 with proteins such as VEGF and MMPs is significant in the context of tumor angiogenesis and metastasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Integrin alpha-V/beta-3 (ITGAV : ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B : ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV : ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415, PubMed : 24789099). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV : ITGB3 act as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428). Integrin ITGA2B : ITGB3 is also the receptor of the erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717).. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Coxsackievirus A9.. (Microbial infection) Acts as a receptor for Hantaan virus.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Cytomegalovirus/HHV-5.. (Microbial infection) Integrin ITGA5 : ITGB3 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGAV : ITGB3 acts aP05556s a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGB3

대체 명칭 보기

CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports. Medicine 5:101852 PubMed39657664

2024

Lurbinectedin sensitizes PD-L1 blockade therapy by activating STING-IFN signaling in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Subhamoy Chakraborty,Utsav Sen,Kedwin Ventura,Vrinda Jethalia,Charles Coleman,Subhasree Sridhar,Avisek Banerjee,Hilal Ozakinci,Yazhini Mahendravarman,Konrad Snioch,Elisa de Stanchina,Misty D Shields,Lewis E Tomalin,Deniz Demircioglu,Theresa A Boyle,Anna Tocheva,Dan Hasson,Triparna Sen

Cells 13: PubMed39273058

2024

The Role of αvβ3 Integrin in Lamina Cribrosa Cell Mechanotransduction in Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mustapha Irnaten,Ellen Gaynor,Colm O'Brien

The Journal of pathology 257:198-217 PubMed35107828

2022

Multisystem screening reveals SARS-CoV-2 in neurons of the myenteric plexus and in megakaryocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Gray-Rodriguez,Melanie P Jensen,Maria Otero-Jimenez,Brian Hanley,Olivia C Swann,Patrick A Ward,Francisco J Salguero,Nadira Querido,Ildiko Farkas,Elisavet Velentza-Almpani,Justin Weir,Wendy S Barclay,Miles W Carroll,Zane Jaunmuktane,Sebastian Brandner,Ute Pohl,Kieren Allinson,Maria Thom,Claire Troakes,Safa Al-Sarraj,Magdalena Sastre,Djordje Gveric,Steve Gentleman,Candice Roufosse,Michael Osborn,Javier Alegre-Abarrategui

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 3 antibody [SP324]

Multiplex immunohistochemistry - Anti-Integrin beta 3 antibody [SP324] (AB227702)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [SP324] (AB227702)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [SP324] (AB227702)

Western blot - Anti-Integrin beta 3 antibody [SP324] (AB227702)

Western blot - Anti-Integrin beta 3 antibody [SP324] (AB227702)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise