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AB316810

Anti-Integrin beta 4 antibody [EPR24648-46] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Integrin beta 4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Integrin beta 4 antibody [EPR24648-46] - BSA and Azide free (Capture) (AB316810)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Integrin beta 4 antibody [EPR24648-46] - BSA and Azide free (Capture) (AB316810)

Sandwich ELISA of ab316809 with the capture antibody ab316810 dilution at 2 ug/mL and detector antibody ab316811 dilution at 0.5 ug/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24648-46

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB314597

Human Integrin beta 4 ELISA Kit - Extracellular

SimpleStep

Sandwich ELISA - Human Integrin beta 4 ELISA Kit - Extracellular (AB314597)

  • 0
  • 0
Sample Prep & Detection Kits

AB236553

Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (AB236553)

  • 5
  • 5
Sample Prep & Detection Kits

AB269893

Latex Conjugation Kit - 400nm Red

Functional Studies - Latex Conjugation Kit - 400nm Red (AB269893)

  • 0
  • 0
Primary Antibodies

AB259692

Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector) (AB259692)

  • 0
  • 0
Primary Antibodies

AB278316

Anti-SGK1 antibody [EPR23300-83] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-SGK1 antibody [EPR23300-83] - BSA and Azide free (Detector) (AB278316)

  • 0
  • 0
Primary Antibodies

AB278320

Anti-IDO-2 antibody [EPR23690A-48] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IDO-2 antibody [EPR23690A-48] - BSA and Azide free (Capture) (AB278320)

  • 0
  • 0
Primary Antibodies

AB316988

Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-155] - BSA and Azide free (Detector)

Recombinant|RabMAb

Sandwich ELISA - Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-155] - BSA and Azide free (Detector) (AB316988)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab316810 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Integrin beta 4. The recommended pair for sandwich ELISA is:

Capture: ab316810, Human Integrin beta 4 Capture Antibody (unconjugated)

Detector: ab316811, Human Integrin beta 4 Detector Antibody (unconjugated)

The reference range value is 15.63-2000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.
Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).
See full target information ITGB4

대체 명칭 보기

CD104, Integrin beta-4, GP150, ITGB4
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Product promise

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