Anti-Interferon gamma antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody (AB25101)

ab25101 staining Human tonsil. Staining is localised to the cytoplasm and protein is secreted.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be

• sELISA

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Sandwich ELISA - Anti-Interferon gamma antibody (AB25101)

Standard curve for Interferon gamma (analyte : ab51240); dilution range 1pg/ml to 1μg/ml using capture antibody ab25014 at 5μg/ml and detector antibody ab25101 at 0.5μg/ml.

• WB

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Western blot - Anti-Interferon gamma antibody (AB25101)

All lanes:

Western blot - Anti-Interferon gamma antibody (ab25101) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Interferon gamma protein (Tag Free) (<a href='/ko/products/proteins-peptides/recombinant-human-interferon-gamma-protein-ab51240'>ab51240</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 19 kDa

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Exposure time: 30s