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AB237509

Anti-Intersectin-2 antibody

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Rabbit Polyclonal Intersectin-2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITSN2 aa 900 to C-terminus.

대체 명칭 보기

KIAA1256, SH3D1B, SWAP, ITSN2, Intersectin-2, SH3 domain-containing protein 1B, SH3P18, SH3P18-like WASP-associated protein

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Intersectin-2 antibody (AB237509)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Intersectin-2 antibody (AB237509)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Intersectin-2 using ab237509 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITSN2 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZM3

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보완 제품

Primary Antibodies

AB118262

Anti-Intersectin 1 antibody

Western blot - Anti-Intersectin 1 antibody (AB118262)

  • 3
  • 2
Primary Antibodies

AB57963

Anti-FMNL2 antibody [2B4]

Western blot - Anti-FMNL2 antibody [2B4] (AB57963)

  • 0
  • 0
Primary Antibodies

AB51243

Anti-ARPC5/p16 ARC antibody [EP1551Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

  • 5
  • 5
Primary Antibodies

AB11826

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

  • 4
  • 16
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity greater than 95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Intersectin-2 also known as ITSNT2 is a multi-domain scaffold protein involved in endocytosis and signal transduction. It has a molecular mass of approximately 194 kDa. Intersectin-2 helps form dynamic protein complexes by acting as an adaptor facilitating protein-protein interactions. This protein mainly expressed in the nervous system enabling synaptic vesicle recycling important for neurotransmission. It contains multiple EH (Eps15 Homology) domains SH3 (Src homology 3) domains and a coiled-coil domain all contributing to its versatile binding capabilities.
Biological function summary

The sophisticated molecular architecture of Intersectin-2 allows it to participate in essential cellular processes like endocytosis and actin cytoskeleton remodeling. It functions by interacting with proteins such as Dynamin Eps15 and Synaptojanin forming complexes necessary for clathrin-mediated endocytosis. Moreover its presence in the nervous system is significant for neuronal communication and plasticity by influencing synaptic vesicle trafficking and recycling. This activity highlights its role in modulating excitatory and inhibitory signals at synapses.

Pathways

Intersectin-2 plays an important role in the endocytic pathways and is a part of the Ras-MAPK signaling cascade. In the endocytic pathway Intersectin-2 interacts with several proteins like Clathrin and AP-2 complex facilitating vesicle formation and internalization of receptor-ligand complexes. In the Ras-MAPK signaling pathway it may regulate downstream signaling events by interacting with components such as SOS influencing cell growth and differentiation. These interactions link it to broader cellular processes like receptor-mediated signaling and cell survival.

Malfunction of Intersectin-2 links to neurological conditions such as Alzheimer's disease and epilepsy. In Alzheimer's disease altered Intersectin-2 expression correlates with dysfunctional endocytic activity and synaptic failure key features in disease progression. Furthermore in epilepsy interactions with proteins like Dynamin and Synaptojanin imply that disruptions in Intersectin-2's function may affect neuronal excitability and neurotransmitter release. Understanding these associations provides insights into potential therapeutic targets for these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information ITSN2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncotarget 11:2906-2918 PubMed32774771

2020

Biomarker analysis to predict the pathological response to neoadjuvant chemotherapy in locally advanced gastric cancer: An exploratory biomarker study of COMPASS, a randomized phase II trial.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Oshima,Takaki Yoshikawa,Yohei Miyagi,Satoshi Morita,Michio Yamamoto,Kazuaki Tanabe,Kazuhiro Nishikawa,Yuichi Ito,Takanori Matsui,Yutaka Kimura,Tomoyuki Yokose,Yukihiko Hiroshima,Toru Aoyama,Tsutomu Hayashi,Takashi Ogata,Haruhiko Cho,Yasushi Rino,Munetaka Masuda,Akira Tsuburaya,Junichi Sakamoto
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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