AB53112

Anti-Involucrin antibody

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(7 리뷰들)

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(60 제품이 사용된 논문 )

Anti-Involucrin antibody (ab53112) is a rabbit polyclonal antibody detecting Involucrin in Western Blot, IHC-P, ICC/IF, ELISA. Suitable for Human.

- Over 40 publications
- Trusted since 2007

대체 명칭 보기

Involucrin, IVL

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (AB53112)

IHC-P

AbReview28420****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (AB53112)

ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (AB53112)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (AB53112)

ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ELISA, WB, IHC-P

Immunogen

Synthetic Peptide within Human IVL. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB181980

Anti-Involucrin antibody [EPR13054]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (AB181980)

5
1

Primary Antibodies

AB234403

Anti-Involucrin antibody [IVRN/2113R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [IVRN/2113R] (AB234403)

0
0

Primary Antibodies

AB68

Anti-Involucrin antibody [SY5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (AB68)

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9

Primary Antibodies

AB240256

Anti-Involucrin antibody [EPR13054] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] - BSA and Azide free (AB240256)

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1

보완 제품

Primary Antibodies

AB85679

Anti-Loricrin antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody (AB85679)

5
11

Primary Antibodies

AB76318

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

5
14

Primary Antibodies

AB119695

Anti-Cytokeratin 14 antibody [SP53]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [SP53] (AB119695)

5
6

Primary Antibodies

AB221155

Anti-Filaggrin antibody [EPR21892]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [EPR21892] (AB221155)

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3

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Involucrin antibody (ab53112) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human samples.

What is the molecular weight of Involucrin?
Anti-Involucrin (ab53112) specifically detects a band for Involucrin (UniProt: P07476) at a molecular weight of 68kDa.

Trusted by the scientific community
Anti-Involucrin (ab53112) was first used in a scientific publication in 2007 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Involucrin (ab53112) has over 5 independent reviews from customers.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab53112 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.

Biological function summary

Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.

Pathways

Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.

Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

See full target information IVL

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제품이 사용된 논문 (60)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bioengineering & translational medicine 10:e70003 PubMed40708979

2025

Carboxymethyl cellulose-polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Hyun Lee,You Bin Lee,Hyun Su Park,Young-Ju Jang,Youn Chul Kim,Suk Ho Bhang

Cells 14: PubMed40710298

2025

Bioengineering a Human Dermal Equivalent Using Induced Pluripotent Stem Cell-Derived Fibroblasts to Support the Formation of a Full-Thickness Skin Construct.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Smith,David Bunton,Michael Finch,Stefan Przyborski

Scientific reports 15:662 PubMed40118897

2025

Dysregulation of STS in keratinocytes promotes calcium signaling and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Tae-Uk Kwon,Yeo-Jung Kwon,Hyemin Park,Yoon-Ji Kang,Young-Jin Chun

Molecular medicine reports 31: PubMed40084708

2025

Effects of natural killer cell‑conditioned medium on UVB‑induced photoaging in human keratinocytes and a human reconstructed skin model.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Ok Lee,Jung Min Lee,Yujin Kim,A Yeon Park,Daewon Yoon,Su Young Kim,Jihye Heo,Seungryel Han,Hyungjin Nam,Hye Jin Shin,Kyeongsoo Jeong,Minju Im,Beom Joon Kim

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70323 PubMed39812615

2025

A noncanonical-GPRC5A signaling regulates keratinocyte adhesion and migration by nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Chanteloube,Choua Ya,Gabrielle Le Provost,Aurore Berthier,Cindy Dieryckx,Sandrine Vadon-Le Goff,Florence Nadal,Bérengère Fromy,Romain Debret

Frontiers in immunology 15:1483000 PubMed39790997

2025

Beneficial effects of ultrafine bubble shower on a mouse model of atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ayaki Matsumoto,Hisayoshi Imanishi,Mika Yamanaka-Takaichi,Masateru Hirae,Daisuke Tsuruta,Kozo Nakai

iScience 27:111093 PubMed39502293

2024

OBP2A regulates epidermal barrier function and protects against cytotoxic small hydrophobic molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Shinobu Nakanishi,Tatsuya Hasegawa,Katsuyuki Maeno,Akira Motoyama,Mitsuhiro Denda

Journal of materials chemistry. B 12:11110-11122 PubMed39466636

2024

Controlled therapeutic cholesterol delivery to cells for the proliferation and differentiation of keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Berniak,Ahmadreza Moradi,Agata Lichawska-Cieslar,Weronika Szukala,Jolanta Jura,Urszula Stachewicz

Cells 13: PubMed38727296

2024

JAK/STAT Inhibition Normalizes Lipid Composition in 3D Human Epidermal Equivalents Challenged with Th2 Cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Flori,Alessia Cavallo,Sarah Mosca,Daniela Kovacs,Carlo Cota,Marco Zaccarini,Anna Di Nardo,Grazia Bottillo,Miriam Maiellaro,Emanuela Camera,Giorgia Cardinali

International journal of molecular sciences 24: PubMed38139209

2023

Targeting Fatty Acid Amide Hydrolase Counteracts the Epithelial-to-Mesenchymal Transition in Keratinocyte-Derived Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Kovacs,Enrica Flori,Emanuela Bastonini,Sarah Mosca,Emilia Migliano,Carlo Cota,Marco Zaccarini,Stefania Briganti,Giorgia Cardinali

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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