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AB184969

Anti-Involucrin antibody [EPR13060(N)] - C-terminal

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Involucrin antibody. C-terminal. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

대체 명칭 보기

Involucrin, IVL

3 이미지
Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

Intracellular flow cytometric analysis of Involucrin expression in paraformaldehyde-fixed HACAT cells using ab184969 at 1/40 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)
  • WB

Supplier Data

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

All lanes:

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/10000 dilution

All lanes:

Human fetal skin tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa

false

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)
  • WB

Supplier Data

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

All lanes:

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/50000 dilution

All lanes:

HT-1080 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13060(N)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB227530

Anti-Involucrin antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody - N-terminal (AB227530)

  • 0
  • 0
Primary Antibodies

AB234403

Anti-Involucrin antibody [IVRN/2113R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [IVRN/2113R] (AB234403)

  • 0
  • 0
Primary Antibodies

AB20202

Anti-Involucrin antibody [SY8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (AB20202)

  • 0
  • 0
Primary Antibodies

AB181980

Anti-Involucrin antibody [EPR13054]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (AB181980)

  • 5
  • 1
Primary Antibodies

AB240256

Anti-Involucrin antibody [EPR13054] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] - BSA and Azide free (AB240256)

  • 5
  • 1

보완 제품

Primary Antibodies

AB137533

Anti-Loricrin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody (AB137533)

  • 0
  • 0
Primary Antibodies

AB76318

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

  • 5
  • 14
Primary Antibodies

AB119695

Anti-Cytokeratin 14 antibody [SP53]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [SP53] (AB119695)

  • 5
  • 6
Primary Antibodies

AB221155

Anti-Filaggrin antibody [EPR21892]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-Filaggrin antibody [EPR21892] (AB221155)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.
Biological function summary

Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.

Pathways

Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.

Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
See full target information IVL
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell chemical biology 26:1365-1379.e22 PubMed31422906

2019

Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsey N Lamb,Daniel Bsteh,Sarah N Dishman,Hagar F Moussa,Huitao Fan,Jacob I Stuckey,Jacqueline L Norris,Stephanie H Cholensky,Dongxu Li,Jingkui Wang,Cari Sagum,Benjamin Z Stanton,Mark T Bedford,Kenneth H Pearce,Terry P Kenakin,Dmitri B Kireev,Gang Greg Wang,Lindsey I James,Oliver Bell,Stephen V Frye

Journal of cellular biochemistry 120:9213-9229 PubMed30556162

2018

Peroxisome proliferator-activated receptor-α agonist and all-trans retinoic acid induce epithelial differentiation of subcutaneous adipose-derived stem cells from debrided burn skin.

Applications

Unspecified application

Species

Unspecified reactive species

Shanmugasundaram Natesan,Nicole L Wrice,Robert J Christy
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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