AB247303

Anti-IPL-1/STK13/Aurora C antibody [EP1011Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IPL-1/STK13/Aurora C antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

AIE2, AIK3, AIRK3, ARK3, STK13, AURKC, Aurora kinase C, Aurora 3, Aurora/IPL1-related kinase 3, Aurora/IPL1/Eg2 protein 2, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-C, ARK-3, Aurora-related kinase 3

1 이미지

Supplier Data

Western blot - Anti-IPL-1/STK13/Aurora C antibody [EP1011Y] - BSA and Azide free (AB247303)

This data was developed using ab46783, the same antibody clone in a different buffer formulation.

All lanes:

HepG2 lysate at 10 µg

Secondary

All lanes:

goat anti rabbit HRP at 1/2000 dilution

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1011Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not recommended in tissue lysate testing.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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제품 세부 정보

ab247303 is the carrier-free version of ab46783.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IPL-1 also known as STK13 or Aurora C is a serine/threonine protein kinase with a molecular mass of approximately 39 kDa. It expresses mainly in testis and ovarian tissue playing a significant role in cell division. As a member of the Aurora kinase family IPL-1 functions mechanistically in regulating the centrosome during cell cycle progression ensuring the proper segregation of chromosomes during mitosis and meiosis. The protein undergoes phosphorylation to activate its kinase activity which is essential for its role in chromatin modification and assembly of microtubules.

Biological function summary

IPL-1 functions in chromosomal alignment and cytokinesis during cell division. It forms part of the chromosomal passenger complex (CPC) along with INCENP Survivin and Borealin. This complex is essential for correcting errors in chromosome segregation. IPL-1's activity helps maintain genomic stability by ensuring that cells divide correctly. Given its role in the chromosomal passenger complex IPL-1 is critical in monitoring and controlling the accurate distribution of genetic material between daughter cells.

Pathways

IPL-1 is intricately involved in cellular processes like the mitotic spindle checkpoint and the regulation of the mitotic cell cycle. It interacts with other proteins such as Aurora B which shares similar functions in the mitotic pathway. IPL-1 helps coordinate the activities of these pathways and proteins to ensure orderly cell division. Through these pathways IPL-1 influences the balance of cell proliferation and cell death which is important for normal organismal development and function.

Misregulation of IPL-1 is associated with cancers including ovarian and testicular cancer. Aberrant expression or activation of IPL-1 can lead to chromosomal instability contributing to tumorigenesis. Additionally the overexpression of Aurora kinase family members including IPL-1 often co-occurs with Cyclin B1 dysregulation in cancer cells. The connection between IPL-1 and Cyclin B1 highlights potential therapeutic targets for cancer treatment as researchers continue to explore strategies for inhibiting IPL-1 to restore normal cell cycle control in tumor cells.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Also plays a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.

See full target information AURKC

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IPL-1/STK13/Aurora C antibody [EP1011Y] - BSA and Azide free

Western blot - Anti-IPL-1/STK13/Aurora C antibody [EP1011Y] - BSA and Azide free (AB247303)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise