Anti-IPS1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-IPS1 antibody (AB240581)

IPS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, using ab240581 at 6 μg per reaction. For western blotting, ab240581 was used at 0.4 μg/ml.

Lane 1 : ab240581 used for IP at 6 μg per reaction.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-IPS1 antibody (ab240581)

Predicted band size: 160 kDa

false

• WB

Supplier Data

Western blot - Anti-IPS1 antibody (AB240581)

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-IPS1 antibody (ab240581) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Predicted band size: 160 kDa

true

Exposure time: 30s