AB192249

Anti-IRAK2 antibody [EPR16530]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IRAK2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

Interleukin-1 receptor-associated kinase-like 2, IRAK-2, IRAK2

4 이미지

Flow Cytometry (Intracellular) - Anti-IRAK2 antibody [EPR16530] (AB192249)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IRAK2 antibody [EPR16530] (AB192249)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed MOLT4cells labeling IRAK2 with ab192249 at 1/220 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] (AB192249)

Western blot analysis of IRAK2 in MOLT4 cell lysates immunoprecipitated using ab192249 at 1/60 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] (ab192249)

Predicted band size: 69 kDa

false

Supplier Data

Western blot - Anti-IRAK2 antibody [EPR16530] (AB192249)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/1000 dilution

Lane 1:

THP1 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 69 kDa

false

Supplier Data

Western blot - Anti-IRAK2 antibody [EPR16530] (AB192249)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/10000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 69 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16530

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/220", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IRAK2 also known as Interleukin-1 Receptor-Associated Kinase 2 plays a central role in the immune response. This protein has a molecular mass of approximately 68 kDa. It is broadly expressed in various tissues particularly in those related to the immune system including the spleen thymus and peripheral blood leukocytes. IRAK2 is a part of the IRAK family which includes other kinases like IRAK1 IRAK3 and IRAK4 sharing structural similarities.

Biological function summary

The IRAK2 protein is critical for transducing signals from interleukin-1 receptors (IL-1R) and Toll-like receptors (TLR) as part of the innate immune response. It does not work alone but as part of the Myddosome complex. This complex formation is essential for signal propagation leading to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinases (MAPKs). Activation of these pathways initiates inflammatory and immune processes enabling the body to respond to pathogens.

Pathways

IRAK2 integrates into the Toll-like receptor signaling pathway acting as a link between upstream receptor activation and downstream transcription factors. It has strong interactions with other key immune components like MyD88 and TRAF6 within these pathways. Through these links IRAK2 contributes to the orchestration of a coordinated immune defense facilitating the body's capacity to manage microbial infections while maintaining regulatory checks to prevent excessive inflammation.

Defects or dysregulation in IRAK2 function can lead to immune-related diseases. For instance aberrant IRAK2 activity associates with rheumatoid arthritis where it drives inflammatory processes contributing to joint damage. Moreover its involvement in the immune signaling network makes it relevant in sepsis a condition resulting from an overwhelming immune response to infection. In these disorders IRAK2 works in conjunction with other proteins such as IRAK1 and TRAF6 influencing disease progression and symptom manifestation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.

See full target information IRAK2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Genome medicine 13:58 PubMed33853662

2021

PRMT1-mediated H4R3me2a recruits SMARCA4 to promote colorectal cancer progression by enhancing EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yao,Tao Gui,Xiangwei Zeng,Yexuan Deng,Zhi Wang,Ying Wang,Dongjun Yang,Qixiang Li,Peipei Xu,Ruifeng Hu,Xinyu Li,Bing Chen,Jin Wang,Ke Zen,Haitao Li,Melissa J Davis,Marco J Herold,Hua-Feng Pan,Zhi-Wei Jiang,David C S Huang,Ming Liu,Junyi Ju,Quan Zhao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRAK2 antibody [EPR16530]

Flow Cytometry (Intracellular) - Anti-IRAK2 antibody [EPR16530] (AB192249)

Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] (AB192249)

Western blot - Anti-IRAK2 antibody [EPR16530] (AB192249)

Western blot - Anti-IRAK2 antibody [EPR16530] (AB192249)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise