AB216132

Anti-IRAKM antibody [5C3D6] - C-terminal

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Mouse Monoclonal IRAKM antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IRAK3 aa 450-600.

대체 명칭 보기

Interleukin-1 receptor-associated kinase 3, IRAK-3, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, IRAK-M, IRAK3

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-IRAKM antibody [5C3D6] - C-terminal (AB216132)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRAKM antibody [5C3D6] - C-terminal (AB216132)

Immunofluorescent analaysis of A549 cells labeling IRAKM with ab216132 at 1/100 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Supplier Data

Western blot - Anti-IRAKM antibody [5C3D6] - C-terminal (AB216132)

All lanes:

Western blot - Anti-IRAKM antibody [5C3D6] - C-terminal (ab216132) at 1/500 dilution

Lane 1:

non-transfected HEK293 cell lysate

Lane 2:

Human IRAKM (aa: 454-596)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-IRAKM antibody [5C3D6] - C-terminal (AB216132)

All lanes:

Western blot - Anti-IRAKM antibody [5C3D6] - C-terminal (ab216132) at 1/500 dilution

All lanes:

Human IRAKM (aa: 454-596) recombinant protein

Predicted band size: 68 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

5C3D6

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human IRAK3 aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

Q9Y616

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/400", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from tissue culture supernatant.

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The IRAK-M protein also known as IRAKM or interleukin-1 receptor-associated kinase M is a member of the IRAK family and has a molecular mass of approximately 65 kDa. It plays an important role in the immune response regulation. This protein is expressed in monocytes and macrophages but it is also found in other immune cells. Unlike other IRAKs IRAK-M acts as a negative regulator helping to ensure the immune system does not overreact.

Biological function summary

IRAK-M inhibits the signaling pathways that lead to the activation of inflammatory responses. It predominantly affects signaling initiated by toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) preventing excessive inflammatory cytokine production. IRAK-M lacks kinase activity unlike other IRAK family members so it functions as a regulatory molecule rather than participating in kinase-mediated catalysis. It does not form part of a larger complex but interacts directly with other signaling components in these pathways.

Pathways

IRAK-M impacts TLR and IL-1 signaling pathways. These pathways are important in the innate immune system's response to pathogens and inflammation. IRAK-M interacts with proteins such as MyD88 and TNF receptor-associated factor 6 (TRAF6) reducing the downstream production of pro-inflammatory cytokines. This modulation helps to maintain immune system balance and prevent chronic inflammation.

IRAK-M is involved in conditions such as sepsis and autoimmune diseases. In sepsis IRAK-M's alteration can lead to an inadequate immune response influencing the body's ability to control infection. In autoimmune diseases abnormal IRAK-M expression may result in regulatory failures leading to persistent inflammation and tissue damage. IRAK-M's interaction with other proteins like MyD88 suggests its pivotal role in these disorders where misregulated pathways can contribute to disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed : 10383454, PubMed : 29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed : 29686383).

See full target information IRAK3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 26: PubMed41009720

2025

p.N370S Mutation Influences the Morphology and Lipid Composition of Extracellular Vesicles in Blood Plasma from Patients with Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana S Usenko,Alena E Kopytova,Artem D Izyumchenko,Darya G Kulabukhova,Artemiy S Silantyev,Victoria D Kazakova,Katerina S Basharova,Anastasia I Bezrukova,Luiza A Garaeva,Evgeny B Pichkur,Alexandra V Artynyuk,Irina V Miliukhina,Alla A Timofeeva,Valentina V Miroshnikova,Stanislav N Naryzhny,Anton K Emelyanov,Natalya B Zakharzhevskaya,Andrey L Konevega,Tatiana A Shtam,Sofya N Pchelina

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRAKM antibody [5C3D6] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-IRAKM antibody [5C3D6] - C-terminal (AB216132)

Western blot - Anti-IRAKM antibody [5C3D6] - C-terminal (AB216132)

Western blot - Anti-IRAKM antibody [5C3D6] - C-terminal (AB216132)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise