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AB327246

Anti-IRF2 Antibody

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Rabbit Polyclonal IRF2 antibody. Suitable for WB, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IRF2.

대체 명칭 보기

Interferon regulatory factor 2, IRF-2, IRF2

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-IRF2 Antibody (AB327246)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRF2 Antibody (AB327246)

Immunofluorescent staining of human cell line A-431 using ab327246 at 0.25–2 µg/ml, showing localization to nucleoplasm & cytosol.

ChIP - Anti-IRF2 Antibody (AB327246)
  • ChIP

Supplier Data

ChIP - Anti-IRF2 Antibody (AB327246)

ChIP-Exo-Seq composite graph for ab327246 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-IRF2 Antibody (AB327246)
  • WB

Supplier Data

Western blot - Anti-IRF2 Antibody (AB327246)

Western blot analysis in human cell line RT-4 and human cell line U-251 MG using ab327246 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-IRF2 Antibody (ab327246)

Lane 2:

Human cell line RT-4

Lane 3:

Human cell line U-251 MG

Predicted band size: 39.35 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ChIP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IRF2.

P14316

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

DNA-binding transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible genes and regulates their expression (PubMed : 39013473, PubMed : 7687740). Mainly acts as a transcription repressor, repressing expression (PubMed : 39013473). Also acts as an activator for several genes including H4 and IL7 (PubMed : 15226432, PubMed : 9540062). Constitutively binds to the ISRE promoter to activate IL7 (PubMed : 15226432). Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth (PubMed : 9540062). Antagonizes IRF1 transcriptional activation (By similarity).. Isoform 2. Unable to bind to IRF2BP1 and IRF2BP2 corepressors and cannot mediate repression.
See full target information IRF2

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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