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AB50772

Anti-IRF3 antibody [IRF35I218]

4

(3 리뷰들)

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(11 제품이 사용된 논문 )

Mouse Monoclonal IRF3 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IRF3 aa 1-150.
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Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (AB50772)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (AB50772)

Immunofluorescent staining of HeLa cells with a 1/10 dilution of ab50772 as primary antibody and AlexaFluor®488 conjugated Goat Anti-mouse IgG diluted 1/200 as the secondary antibody

Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (AB50772)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (AB50772)

Overlay histogram showing HeLa cells stained with ab50772 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50772, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)
  • WB

Unknown

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg Secondary Anti-mouse IgG at 1/1000 dilution

All lanes:

Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution

All lanes:

Ripa lysate of Hela cells at 500 µg

Secondary

All lanes:

Anti-mouse IgG at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 55 kDa

false

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)
  • WB

Unknown

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

All lanes:

Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/50 dilution

All lanes:

HT-1080 whole cell lysate at 25 µg

Secondary

All lanes:

Anti mouse IgG at 1/2500 dilution

Predicted band size: 47 kDa

Observed band size: 55 kDa

false

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)
  • WB

AbReview65364****

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

All lanes:

Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/2000 dilution

All lanes:

Human THP1 lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti Mouse Secondary Antibody at 1/10000 dilution

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

IRF35I218

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human IRF3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q14653

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대체품

Primary Antibodies

AB209919

PE Anti-IRF3 antibody [EPR2418Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - PE Anti-IRF3 antibody [EPR2418Y] (AB209919)

  • 0
  • 0
Primary Antibodies

AB205443

HRP Anti-IRF3 antibody [EPR2418Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-IRF3 antibody [EPR2418Y] (AB205443)

  • 0
  • 0
Primary Antibodies

AB76493

Anti-IRF3 (phospho S386) antibody [EPR2346]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (AB76493)

  • 4
  • 2
Primary Antibodies

AB68481

Anti-IRF3 antibody [EPR2418Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] (AB68481)

  • 5
  • 2
Primary Antibodies

AB76409

Anti-IRF3 antibody [EP2419Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EP2419Y] (AB76409)

  • 1
  • 1
Primary Antibodies

AB182859

Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free

RabMAb|Recombinant

Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (AB182859)

  • 0
  • 0
Primary Antibodies

AB201809

Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809)

  • 4
  • 1

보완 제품

Assay Kits

AB207210

IRF3 Transcription Factor Assay Kit (Colorimetric)

Functional Studies - IRF3 Transcription Factor Assay Kit (Colorimetric) (AB207210)

  • 0
  • 0
Primary Antibodies

AB181125

Anti-STING antibody [EPR13130]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-STING antibody [EPR13130] (AB181125)

  • 4
  • 1
Primary Antibodies

AB76493

Anti-IRF3 (phospho S386) antibody [EPR2346]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (AB76493)

  • 4
  • 2
Primary Antibodies

AB40676

Anti-NAK/TBK1 antibody [EP611Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-NAK/TBK1 antibody [EP611Y] (AB40676)

  • 5
  • 2
Primary Antibodies

AB109272

Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (AB109272)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purity
Tissue culture supernatant
Purification 관련 사항
Concentrated and dialyzed culture medium (protein free medium) of hybridoma
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1.16% Sodium chloride, 1% BSA, 0.316% Tris HCl
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.
Biological function summary

IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.

Pathways

IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.

IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed : 22394562, PubMed : 24049179, PubMed : 25636800, PubMed : 27302953, PubMed : 31340999, PubMed : 36603579, PubMed : 8524823, PubMed : 39362857). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed : 11846977, PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 32972995, PubMed : 36603579, PubMed : 8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed : 22394562, PubMed : 25636800, PubMed : 27302953, PubMed : 36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed : 16154084, PubMed : 27302953, PubMed : 33440148, PubMed : 36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed : 16846591). In response to Sendai virus infection, is recruited by TOMM70 : HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70 : HSP90AA1 : IRF3 : BAX inducing apoptosis (PubMed : 25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).
See full target information IRF3

대체 명칭 보기

Interferon regulatory factor 3, IRF-3, IRF3
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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

mBio 14:e0033223 PubMed37650650

2023

Human OTUD6B positively regulates type I IFN antiviral innate immune responses by deubiquitinating and stabilizing IRF3.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Hui Zheng,Chunsheng Dong,Sidong Xiong

Nature 604:557-562 PubMed35388221

2022

Activation of STING by targeting a pocket in the transmembrane domain.

Applications

Unspecified application

Species

Unspecified reactive species

Defen Lu,Guijun Shang,Jie Li,Yong Lu,Xiao-Chen Bai,Xuewu Zhang

PLoS pathogens 16:e1009028 PubMed33253291

2020

Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany L Uhlorn,Robert Jackson,Shuaizhi Li,Shauna M Bratton,Koenraad Van Doorslaer,Samuel K Campos

Life science alliance 3: PubMed32661021

2020

Attenuation of cGAS/STING activity during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany L Uhlorn,Eduardo R Gamez,Shuaizhi Li,Samuel K Campos

International journal of oncology 52:1623-1632 PubMed29512705

2018

Interferon regulatory factor 3 mediates Poly(I:C)-induced innate immune response and apoptosis in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yi,Dan Sun,Qian Han,Zhonghui Liu,Zeng Zeng,Yanping Wu,Xiaoyu Chai,Xinmin Liu

Molecular therapy. Methods & clinical development 6:194-206 PubMed28831401

2017

Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Hung Wang,Nenavath Gopal Naik,Lin-Li Liao,Sung-Chan Wei,Yu-Chan Chao

PLoS pathogens 12:e1005967 PubMed27764250

2016

Histone H2B-IFI16 Recognition of Nuclear Herpesviral Genome Induces Cytoplasmic Interferon-β Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jawed Iqbal,Mairaj Ahmed Ansari,Binod Kumar,Dipanjan Dutta,Arunava Roy,Leela Chikoti,Gina Pisano,Sujoy Dutta,Shahrooz Vahedi,Mohanan Valiya Veettil,Bala Chandran

Journal of virology 88:1582-90 PubMed24257594

2013

Direct, interferon-independent activation of the CXCL10 promoter by NF-κB and interferon regulatory factor 3 during hepatitis C virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Brownell,Jacob Bruckner,Jessica Wagoner,Emmanuel Thomas,Yueh-Ming Loo,Michael Gale,T Jake Liang,Stephen J Polyak

The Journal of biological chemistry 286:10225-32 PubMed21245149

2011

Morphine inhibits murine dendritic cell IL-23 production by modulating Toll-like receptor 2 and Nod2 signaling.

Applications

ICC/IF

Species

Mouse

Jinghua Wang,Jing Ma,Rick Charboneau,Roderick Barke,Sabita Roy

Journal of immunology (Baltimore, Md. : 1950) 183:4422-31 PubMed19748983

2009

TLR3 ligand polyinosinic:polycytidylic acid induces IL-17A and IL-21 synthesis in human Th cells.

Applications

WB

Species

Human

Christian K Holm,Charlotte C Petersen,Malene Hvid,Line Petersen,Søren R Paludan,Bent Deleuran,Marianne Hokland
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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