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AB313466

Anti-IRF5 antibody [RP23040171]

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Rabbit Recombinant Multiclonal IRF5 antibody. Suitable for ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IRF5.

대체 명칭 보기

Interferon regulatory factor 5, IRF-5, IRF5

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-IRF5 Antibody [RP23040171] (AB313466)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRF5 Antibody [RP23040171] (AB313466)

Immunofluorescence was performed on fixed and permeabilized THP-1 cells for detection of endogenous IRF5 using ab313466 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1 : 2000). Panel a) shows representative cells that were stained for detection and localization of IRF5 protein (green), Panel b) is stained for nuclei (blue) using with DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of IRF5. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-IRF5 Antibody [RP23040171] (AB313466)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRF5 Antibody [RP23040171] (AB313466)

Immunofluorescence analysis of IRF5 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ab313466 at 5 ug/ml n 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor®Plus 488 at a dilution of 1 : 2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with with DAPI . F-actin (Panel c : red) was stained with Rhodamine Phalloidin ( 1 : 300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents Jurkat cells having no expression of IRF5. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ChIP - Anti-IRF5 Antibody [RP23040171] (AB313466)
  • ChIP

Supplier Data

ChIP - Anti-IRF5 Antibody [RP23040171] (AB313466)

Enrichment of endogenous IRF5 protein at specific gene loci using ab313466 : Chromatin Immunoprecipitation (ChIP) was performed using ab313466,(5ug) on sheared chromatin from 2 million THP1 cells treated with 10 ng/mL of Leptomycin B for 90 minutes. Normal Rabbit IgG (1 ug) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs of the active IL6, IFNA1, TNF ALPHA promoter regions used as positive control target genes, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

주요 정보

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040171

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP

applications

Immunogen

Synthetic Peptide within Human IRF5. Database link Q13568

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보완 제품

Assay Kits

AB282878

Human IRF5 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IRF5 ELISA Kit (AB282878)

  • 0
  • 0
Primary Antibodies

AB309088

Anti-IRF5 (phospho S446) antibody [EPR26132-254]

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Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (AB309088)

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Primary Antibodies

AB68481

Anti-IRF3 antibody [EPR2418Y]

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Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] (AB68481)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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  • 24
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2.5 µg for 10^6 Cells", "ChIP-species-notes": "<p>2.5 μg x 10^6 cells</p>" } } }
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제품 세부 정보

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IRF5 or Interferon Regulatory Factor 5 is a member of the IRF family of transcription factors. This protein weighs approximately 56 kDa and is expressed in cells of the immune system including B cells dendritic cells and macrophages. IRF5 plays an important role in regulating the transcription of interferon-stimulated genes following a viral infection. It acts by binding to specific DNA sequences initiating transcription processes that are important for the antiviral response.
Biological function summary

IRF5 influences the immune response through the regulation of cytokine production. It activates genes involved in producing pro-inflammatory cytokines such as TNF-α and IL-6 which are important for clearing viral infections and activating immune cells. IRF5 does not usually form part of a protein complex but it interacts closely with TRAF6 and MyD88 for its activation. Its pivotal role in cytokine production highlights its importance in controlling inflammation.

Pathways

IRF5 is an important component of the Toll-like receptor (TLR) signaling pathway and plays a role in the MyD88-dependent pathway. In this context IRF5 gets activated by TLRs interacting with MyD88 and TAK1 to enhance the transcription of important genes for immune response. It is also closely connected to the IRF3 protein which shares similar activation pathways and can similarly initiate an antiviral state.

Defects or dysregulation in IRF5 function are linked to autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE IRF5 influences autoantibody production contributing to disease development. It works alongside other regulatory proteins involved in autoimmunity including IFN-α and STAT4 which also play significant roles in modulating immune responses. Understanding IRF5's role in these conditions could present new opportunities for therapeutic interventions using IRF5 inhibitors.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed : 11303025, PubMed : 15695821, PubMed : 22412986, PubMed : 25326418, PubMed : 32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).
See full target information IRF5
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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