AB238580

Anti-IRG1 antibody

Name: Anti-IRG1 antibody
Brand: Abcam Limited
SKU: AB238580
Rating: 4 (1 reviews)

(1 리뷰)

(2 제품이 사용된 논문 )

Rabbit Polyclonal IRG1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACOD1 aa 50-300.

대체 명칭 보기

IRG1, ACOD1, Cis-aconitate decarboxylase, CAD, Aconitate decarboxylase, Aconitate decarboxylase 1, Cis-aconitic acid decarboxylase, Immune-responsive gene 1 protein

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)

Paraffin-embedded human appendix tissue stained for IRG1 using ab238580 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)

Paraffin-embedded human glioma tissue stained for IRG1 using ab238580 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACOD1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

A6NK06

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IRG1 also known as Immune-Responsive Gene 1 protein has a molecular weight of approximately 50 kDa. This protein is expressed largely in immune cells such as macrophages and dendritic cells. Mechanically IRG1 catalyzes the production of itaconate from cis-aconitate during the citric acid cycle an action that has gained significant interest due to its immunomodulatory effects. The localization of IRG1 in immune cells suggests a role in the response to pathogenic threats and regulation of inflammation.

Biological function summary

IRG1 is important for immune response regulation by producing itaconate an anti-inflammatory metabolite. Itaconate acts on innate immune signaling pathways to dampen excessive inflammatory responses. Though IRG1 is not part of a larger protein complex it interacts closely with cellular metabolism. This interaction plays a significant role in metabolic reprogramming during infections thereby influencing cytokine production and the activation state of cells.

Pathways

IRG1 plays a significant role in the tricarboxylic acid (TCA) cycle and is associated with the innate immune response. The protein shifts cellular metabolism towards cataplerosis to accommodate the need for itaconate production. IRG1 is connected to regulatory proteins such as the transcription factor NRF2 which mediates antioxidative responses. Itaconate a product of IRG1 activity can modulate pathways like the NF-kB signaling pathway attenuating inflammatory responses.

IRG1 has been implicated in atherosclerosis and tuberculosis. In atherosclerosis IRG1 contributes to the anti-inflammatory state within macrophages which can alter plaque formation. Through itaconate IRG1 interacts with inflammasome components potentially impacting the pathology of the disease. In tuberculosis IRG1-induced itaconate production helps in macrophage defense against Mycobacterium tuberculosis. Additionally pathways involving IRG1 often intersect with proteins like HIF-1α under hypoxic conditions relevant to the progression and management of these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response (PubMed : 23609450, PubMed : 23610393, PubMed : 31548418, PubMed : 35662396). Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages (PubMed : 23609450). Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB (PubMed : 23610393, PubMed : 35662396). Involved in antiviral response following infection by flavivirus in neurons : ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). Plays a role in the embryo implantation (By similarity).

See full target information ACOD1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Radiology and oncology 58:67-77 PubMed38378037

2024

RAD54B promotes gastric cancer cell migration and angiogenesis via the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianchao Li,Hui Geng,Xin Li,Shenshan Zou,Xintao Xu

The Journal of clinical investigation 134: PubMed38085578

2023

Itaconate suppresses atherosclerosis by activating a Nrf2-dependent antiinflammatory response in macrophages in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianrui Song,Yanling Zhang,Ryan A Frieler,Anthony Andren,Sherri Wood,Daniel J Tyrrell,Peter Sajjakulnukit,Jane C Deng,Costas A Lyssiotis,Richard M Mortensen,Morgan Salmon,Daniel R Goldstein

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRG1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise