AB119044

Anti-ITM2B antibody [OTI1C11]

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(2 제품이 사용된 논문 )

Mouse Monoclonal ITM2B antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ITM2B.

대체 명칭 보기

BRI, BRI2, ITM2B, Integral membrane protein 2B, Immature BRI2, Protein E25B, Transmembrane protein BRI, imBRI2, Bri

4 이미지

Flow Cytometry (Intracellular) - Anti-ITM2B antibody [OTI1C11] (AB119044)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ITM2B antibody [OTI1C11] (AB119044)

ab119044 at 1/100 dilution staining ITM2B in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ITM2B antibody [OTI1C11] (AB119044)

ab119044 at 1/100 dilution staining ITM2B in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2B antibody [OTI1C11] (AB119044)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2B antibody [OTI1C11] (AB119044)

ab119044, at 1/150 dilution, staining ITM2B in paraffin embedded Human kidney by Immunohistochemistry.

Unknown

Western blot - Anti-ITM2B antibody [OTI1C11] (AB119044)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202377)

All lanes:

Western blot - Anti-ITM2B antibody [OTI1C11] (ab119044) at 1/2000 dilution

Lane 1:

HEK293T lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T lysate transfected with pCMV6-ENTRY ITM2B cDNA at 5 µg

Predicted band size: 30 kDa,55 kDa

Observed band size: 75 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1C11

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ITM2B.

Q9Y287

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody." } } }

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제품 세부 정보

Dilute in PBS (pH7.3) before use.

The clone number has been updated from 1C11 to OTI1C11, both clone numbers name the same clone.

This product was changed from ascites to tissue culture supernatant on 5^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ITM2B also known as Integral Membrane Protein 2B or BRI2 is a transmembrane protein weighing approximately 30 kDa. This protein is expressed widely in human tissues showing particularly high levels in the central nervous system and brain. ITM2B functions mechanically by playing a role in protein processing and trafficking owing to its transmembrane nature. With an integral role in cellular functions ITM2B interacts with various cellular components associated with membrane-related activities.

Biological function summary

ITM2B engages in several vital cellular processes such as regulation of amyloid precursor protein (APP) processing. ITM2B forms part of a complex with other proteins involved in APP cleavage which is significant for maintaining cell homeostasis. It also influences neuronal functions through its effects on synaptic plasticity and could impact cognitive abilities. The protein's presence in brain regions ties ITM2B closely to neural activities where protein interactions are essential for cellular communication.

Pathways

ITM2B exerts influence on critical processes in amyloidogenic pathways contributing to the modulation of amyloid-beta production. This protein directly interacts with other players like APP and presenilin 1 within this pathway affecting the levels of amyloids and ultimately impacting neural integrity. Additionally ITM2B participates in Wnt signaling pathways linking it to cellular differentiation and proliferation where it modulates interactions with proteins that convey Wnt signals such as beta-catenin.

ITM2B is directly linked to familial British dementia and familial Danish dementia both neurodegenerative conditions. These diseases involve mutations in the ITM2B gene leading to abnormal protein aggregates that disrupt normal brain function. ITM2B's relationship with amyloid-beta and presenilin 1 also connects it to Alzheimer's disease pathogenesis where these proteins play roles in amyloid plaque formation a hallmark of Alzheimer's. Studies focus on targeting ITM2B interactions and processing to develop therapies for these conditions highlighting its potential as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a regulatory role in the processing of the amyloid-beta A4 precursor protein (APP) and acts as an inhibitor of the amyloid-beta peptide aggregation and fibrils deposition. Plays a role in the induction of neurite outgrowth. Functions as a protease inhibitor by blocking access of secretases to APP cleavage sites.. Mature BRI2 (mBRI2) functions as a modulator of the amyloid-beta A4 precursor protein (APP) processing leading to a strong reduction in the secretion of secretase-processed amyloid-beta protein 40 and amyloid-beta protein 42.. Bri23 peptide prevents aggregation of APP amyloid-beta protein 42 into toxic oligomers.

See full target information ITM2B

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of clinical investigation 133: PubMed37014703

2023

Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Braun,Ahmed Abed,Dominik Sellung,Manuel Rogg,Mathias Woidy,Oysten Eikrem,Nicola Wanner,Jessica Gambardella,Sandra D Laufer,Fabian Haas,Milagros N Wong,Bernhard Dumoulin,Paula Rischke,Anne Mühlig,Wiebke Sachs,Katharina von Cossel,Kristina Schulz,Nicole Muschol,Sören W Gersting,Ania C Muntau,Oliver Kretz,Oliver Hahn,Markus M Rinschen,Michael Mauer,Tillmann Bork,Florian Grahammer,Wei Liang,Thorsten Eierhoff,Winfried Römer,Arne Hansen,Catherine Meyer-Schwesinger,Guido Iaccarino,Camilla Tøndel,Hans-Peter Marti,Behzad Najafian,Victor G Puelles,Christoph Schell,Tobias B Huber

American journal of human genetics 99:115-24 PubMed27346687

2016

DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Hemanth Tummala,Amanda J Walne,Mike Williams,Nicholas Bockett,Laura Collopy,Shirleny Cardoso,Alicia Ellison,Rob Wynn,Thierry Leblanc,Jude Fitzgibbon,David P Kelsell,David A van Heel,Elspeth Payne,Vincent Plagnol,Inderjeet Dokal,Tom Vulliamy

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITM2B antibody [OTI1C11]

Flow Cytometry (Intracellular) - Anti-ITM2B antibody [OTI1C11] (AB119044)

Flow Cytometry (Intracellular) - Anti-ITM2B antibody [OTI1C11] (AB119044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2B antibody [OTI1C11] (AB119044)

Western blot - Anti-ITM2B antibody [OTI1C11] (AB119044)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise