AB322107

Anti-Jagged 2/JAG2 antibody [EPR29026-90]

Recombinant
RabMAb
KO Validated
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Knockout Tested Rabbit Recombinant Monoclonal Jagged 2/JAG2 antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.

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Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab322107 was shown to bind specifically to JAG2. Target of interest was observed at 180 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in JAR2 knockout cell line (lane 2) (lane 2, knockout cell line ab265966.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (ab322107) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 60 µg

Lane 2:

Western blot - Human JAG2 (Jagged 2) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-jag2-jagged-2-knockout-hela-cell-line-ab265966'>ab265966</a>) at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa,36 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Raji (PMID : 20133585).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (ab322107) at 1/1000 dilution

Lane 1:

SR (human pleural effusion lymphoblast) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa,124 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

Exposure time : Lane 1 : 114 seconds, Lane2 : 26 seconds

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (ab322107) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 60 µg

Lane 2:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa

false

Supplier Data

Dot Blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Dot blot analysis of Jagged 2/JAG2 using ab322107 at 1 : 1000 (0.529 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human JAG2 fragment
Lane2 : His-tagged human JAG1

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human JAG1.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (ab322107) at 1/1000 dilution

Lane 1:

His-tagged human JAG2 fragment

Lane 2:

His-tagged human JAG1 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (ab322107) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa

false

Exposure time: 114s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29026-90

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Jagged 2 (JAG2) also known as JAGGED 2 is a membrane-bound ligand that weighs about 135 kDa. It interacts with the Notch family receptors to initiate signaling events that regulate cell fate decisions. JAG2 expresses in various tissues with high levels in neural hematopoietic and muscular systems. Its mechanical function involves binding to the Notch receptor on adjacent cells facilitating proteolytic cleavage and activation of the receptor's intracellular domain which then translocates to the nucleus to influence gene expression.

Biological function summary

The JAG2 protein plays a significant role in development and differentiation processes. It acts as a ligand in the Notch signaling pathway which includes other ligands like Jagged 1 (JAG1) and Delta-like proteins. In this complex JAG2 modulates neurogenesis myogenesis and angiogenesis. By affecting these processes JAG2 contributes to tissue homeostasis and the regulation of stem cells.

Pathways

The Notch signaling pathway involving JAG2 plays an important role in cell-cell communication. JAG2 activity in this pathway impact pathways like T-cell development and bone remodeling. It interacts with proteins like Notch1 and RBP-Jκ forming a pivotal part of the regulatory circuit that manages cell differentiation and proliferation.

Researchers link JAG2 to various conditions notably cancer and skeletal disorders. Dysregulation of JAG2 in the Notch pathway can contribute to oncogenesis with increased JAG2 expression observed in certain breast cancers. Additionally interactions between JAG2 and the Notch receptors influence bone diseases as alterations in signaling affect bone density and structure. Understanding the role of JAG2 and related proteins in these pathways allows potential therapeutic targeting in these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Putative Notch ligand involved in the mediation of Notch signaling. Involved in limb development (By similarity).

See full target information JAG2

대체 명칭 보기

Protein jagged-2, Jagged2, hJ2, JAG2, Protein jagged-2, Jagged2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Jagged 2/JAG2 antibody [EPR29026-90]

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Dot Blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (AB322107)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise