AB39636

Anti-JAK2 antibody

Name: Anti-JAK2 antibody
Brand: Abcam Limited
SKU: AB39636
Rating: 5 (1 reviews)

(1 리뷰)

(52 제품이 사용된 논문 )

Rabbit Polyclonal JAK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Human JAK2.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (AB39636)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (AB39636)

ab39636 at a 1/50 dilution, staining JAK2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue. Left image shows section without blocking peptide. Right image shows section with blocking peptide.

Supplier Data

Western blot - Anti-JAK2 antibody (AB39636)

All lanes:

Western blot - Anti-JAK2 antibody (ab39636) at 1/500 dilution

Lane 1:

K562 cells at 15 µg

Lane 2:

K562 cells at 15 µg with Blocked with immunising peptide

Predicted band size: 131 kDa

false

IHC-P

AbReview18570****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (AB39636)

Formalin-fixed, paraffin-embedded dog lung tissue stained fo JAK2 using ab39636 at 1/400 dilution in immunohistochemical analysis. Primary antibosy was incubated for 30 minutes at 20°C.

Heat mediated antigen retrieval was performed using 1 mM EDTA buffer pH 8.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human JAK2. The exact immunogen used to generate this antibody is proprietary information.

O60674

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Affinity purified using epitope-specific immunogen.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

JAK2 also known as Janus kinase 2 is a protein tyrosine kinase with a molecular weight of 125 kDa. It plays an essential mechanical role in the signaling pathways by acting as an intermediary between cell surface receptors and intracellular signaling molecules. The JAK2 protein binds to certain cytokine receptors facilitating signal transduction necessary for various cellular responses. JAK2 is expressed in many tissues including hematopoietic cells which are associated with the blood and immune systems.

Biological function summary

JAK2 is important for transmitting signals that dictate cell growth survival and differentiation within the hematopoietic system. It operates bodily functions by forming complexes with specific phosphorylation sites on its associated receptors. Through this formation JAK2 influences the signaling cascade particularly by interacting with other signal transducers and activators where it phosphorylates and becomes activated. This action affects gene transcription directly correlated with cellular proliferation and differentiation processes.

Pathways

The function of JAK2 integrates into the JAK-STAT signaling pathway which is an important pathway in the regulation of immune function growth and development. It works in conjunction with proteins such as STAT3 and STAT5 to transmit signals from cytokine receptors to the nucleus. This pathway critically impacts responses like inflammation and hematopoiesis aligning with its role in precursor proliferation within the bone marrow and various immune cells’ function.

JAK2 has significant implications in conditions like myeloproliferative neoplasms and polycythemia vera. Mutations in the JAK2 gene notably the JAK2 V617F mutation lead to uncontrolled cell division as they disrupt normal signaling mechanisms often resulting in blood cell disorders. In these contexts JAK2 interacts with proteins such as EpoR and MPL which play roles within these disease pathways. Understanding how JAK2 mutations contribute to disease progression offers pathways for targeted therapies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin receptor (MPL/TPOR); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed : 15690087, PubMed : 7615558, PubMed : 9657743, PubMed : 15899890). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed : 15690087, PubMed : 9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain (PubMed : 9657743). Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed : 21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed : 20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed : 21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed : 19783980). Up-regulates the potassium voltage-gated channel activity of KCNA3 (PubMed : 25644777).

See full target information JAK2

대체 명칭 보기

Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2

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제품이 사용된 논문 (52)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Open life sciences 20:20221056 PubMed40321161

2025

Convallatoxin inhibits proliferation and angiogenesis of glioma cells via regulating JAK/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongfei Hao,Yaming Han,Yunfei Bo,Liwen Cao,Huijie Fang,Yufei Zhang,Qingbin Li

Experimental and therapeutic medicine 27:17 PubMed38223328

2024

Triptolide alleviates the development of inflammation in ankylosing spondylitis via the NONHSAT227927.1/JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ding,Jian Liu,Yanqiu Sun,Xiaolu Chen

Experimental and therapeutic medicine 25:231 PubMed37123211

2023

Expression of long non‑coding RNA NONHSAT227927.1 and its effect on the JAK2/STAT3 signaling pathway and inflammation in patients with ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ding,Jian Liu,Yanqiu Sun

BMC cancer 23:179 PubMed36814203

2023

FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xujun Li,Jiangyi Liu

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 42:367-374 PubMed35426800

2022

[Triptolide inhibits inflammatory response and migration of fibroblast like synovial cells in rheumatoid arthritis through the circRNA 0003353/JAK2/STAT3 signaling pathway].

Applications

Unspecified application

Species

Unspecified reactive species

J Wang,J Liu,J Wen,X Wang

Cell death discovery 8:121 PubMed35296654

2022

The possible involvement of circRNA DMNT1/p53/JAK/STAT in gestational diabetes mellitus and preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Dongqin Bao,Chaohui Zhuang,Yan Jiao,Li Yang

BMC complementary medicine and therapies 22:73 PubMed35296317

2022

Chrysoeriol suppresses hyperproliferation of rheumatoid arthritis fibroblast-like synoviocytes and inhibits JAK2/STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ying Wu,Ying-Jie Chen,Xiu-Qiong Fu,Jun-Kui Li,Ji-Yao Chou,Cheng-Le Yin,Jing-Xuan Bai,Ying Wu,Xiao-Qi Wang,Amy Sze-Man Li,Lut Yi Wong,Zhi-Ling Yu

Medical science monitor : international medical journal of experimental and clinical research 28:e934914 PubMed35279667

2022

Curcumol Attenuates Endometriosis by Inhibiting the JAK2/STAT3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Xiao-Bo Nie,Su-Jun Liu,Jiao Liu,Wen-Hui Bian

Diagnostic pathology 16:93 PubMed34689819

2021

CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang Qiu

Clinical and experimental pharmacology & physiology 49:113-121 PubMed34525226

2021

Naringin promotes osteogenesis and ameliorates osteoporosis development by targeting JAK2/STAT3 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Wang,Jie Mao,Yan Chen,Jing Zuo,Lin Chen,Yajing Li,Yingqian Gao,Qibin Lu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JAK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (AB39636)

Western blot - Anti-JAK2 antibody (AB39636)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (AB39636)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (52)

Product promise