Anti-JNK1 antibody [EPR17557]
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
- 각 태그의 뜻
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(84 제품이 사용된 논문 )
Knockout Tested Rabbit Recombinant Monoclonal JNK1 antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Hamster, African green monkey, Xenopus tropicalis, Chicken, Human, Dog samples. Cited in 84 publications.
대체 명칭 보기
JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c
- WB
Lab
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : JNK1 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab199380 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab199380 was shown to recognize JNK1 when JNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and JNK1 knockout samples were subjected to SDS-PAGE. ab199380 and ab8226 (loading control to beta Actin) were diluted to 1/2500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)
Predicted band size: 48 kDa
false
- WB
Supplier Data
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution
Lane 1:
Human fetal heart lysate at 10 µg
Lane 2:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa,54 kDa
false
- WB
Supplier Data
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution
All lanes:
Human fetal brain lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa,54 kDa
false
- WB
Supplier Data
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/10000 dilution
Lane 1:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
U937 (Human histiocytic lymphoma cells) whole cell lysate at 20 µg
Lane 3:
MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4:
Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa,54 kDa
false
- WB
Lab
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
False colour image of Western blot : Anti-JNK1 antibody [EPR17557] staining at 1/2500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab199380 was shown to bind specifically to JNK1. A band was observed at 42/48 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in mapk8 knockout cell line ab277181 (knockout cell lysate ab277223). To generate this image, wild-type and mapk8 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution
Lane 1:
Wild-type U-2 OS cell lysate at 20 µg
Lane 2:
MAPK8 knockout U-2 OS cell lysate at 20 µg
Observed band size: 42-48 kDa
false
- WB
Supplier Data
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution
Lane 1:
Rat brain lysate at 10 µg
Lane 2:
Rat heart lysate at 10 µg
Lane 3:
Rat spleen lysate at 10 µg
Lane 4:
C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 5:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 6:
PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
Lane 7:
NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa,54 kDa
false
- WB
Supplier Data
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution
Lane 1:
MDCK (Canine kidney cell line) whole cell lysates at 10 µg
Lane 2:
COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 10 µg
Lane 3:
UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa,54 kDa
false
- WB
Supplier Data
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Blocking/Dilution buffer : 5% NFDM/TBST.
Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution
Lane 1:
Full length Human JNK1 recombinant protein at 0.01 µg
Lane 2:
Full length Human JNK2 recombinant protein at 0.01 µg
Lane 3:
Full length Human JNK3 recombinant protein at 0.01 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
false
- WB
Supplier Data
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution
Lane 1:
Xenopus (X. tropicalis) muscle lysates at 10 µg
Lane 2:
BHK (Hamster kidney fibroblast cells) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa,54 kDa
false
- WB
CiteAb
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
JNK1 western blot using anti-JNK1 antibody [EPR17557] ab199380. Publication image and figure legend from Zhang, Z., Zhu, L., et al., 2019, Cell Death Dis, PubMed 31296837.
ab199380 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab199380 please see the product overview.
The ERK1/2 signaling pathway was responsible for the effects of CTRP3 on MSCs.a, b Western blot analysis of phospho-Akt/Akt, phospho-ERK1/2/ERK1/2, phospho-AMPK/AMPK, and phospho-JNK/JNK following CTRP3 (n = 3). c, d Effects of U0126 (an ERK1/2 inhibitor) on CTRP3-induced upregulation of MMP9, MT1/2, and SOD2. GAPDH served as a control (n = 4). e–g Effects of ERK1/2 inhibition on MSCs migration and proliferation (n = 4). Images were at original magnification, ×200. (H) CCK-8 assays were used to detect the effects of MMP9 neutralization on CTRP3-induced cell proliferation (n = 5). i, j Effects of ERK1/2 inhibition on apoptosis induced by oxidative stress via TUNEL staining (n = 4). All data are presented as means ± SEMs. *p < 0.05; **p < 0.01; ***p < 0.001
false
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-JNK1 antibody [EPR17557] - BSA and Azide free
Reactivity 정보
제품 세부 정보
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This kinase plays an important role in processes such as inflammation apoptosis and cellular stress responses. JNK1 is not just an isolated enzyme. It forms complexes with other proteins under specific conditions facilitating diverse cellular responses. For example JNK1 activation influences transcription factors like c-Jun by phosphorylating them impacting gene expression related to cell survival and death. This activity establishes JNK1 as a significant player in routine cell functioning and response to external environmental stressors.
Pathways
The kinase is part of the MAPK signaling pathways and the stress-activated protein kinase (SAPK) pathways. These pathways involve multiple signaling cascades important for transmitting extracellular signals into the cellular environment. JNK1 interacts with proteins like MKK4 and MKK7 which are upstream activators and ATF2 a downstream target. This positioning makes JNK1 an essential signaling node that translates extracellular stressors into cellular responses providing adaptability to cells amidst changing conditions.
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타겟 정보
제품이 사용된 논문 (84)
Recent publications for all applications. Explore the 전체 목록 and refine your search
RSC medicinal chemistry : PubMed40236619
2025
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Frontiers in oncology 14:1433256 PubMed39717750
2024
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Cell communication and signaling : CCS 22:478 PubMed39375718
2024
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Chinese medicine 19:128 PubMed39285464
2024
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Cell death & disease 15:395 PubMed38839744
2024
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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 28:239-252 PubMed38682172
2024
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Naunyn-Schmiedeberg's archives of pharmacology 397:6611-6621 PubMed38472369
2024
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Discover oncology 15:45 PubMed38383815
2024
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Experimental and therapeutic medicine 27:55 PubMed38234627
2024
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Experimental and therapeutic medicine 27:8 PubMed38223326
2024
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Product promise
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