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AB199380

Anti-JNK1 antibody [EPR17557]

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(84 제품이 사용된 논문 )

Knockout Tested Rabbit Recombinant Monoclonal JNK1 antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Hamster, African green monkey, Xenopus tropicalis, Chicken, Human, Dog samples. Cited in 84 publications.

대체 명칭 보기

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

10 이미지
Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Lab

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : JNK1 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab199380 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab199380 was shown to recognize JNK1 when JNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and JNK1 knockout samples were subjected to SDS-PAGE. ab199380 and ab8226 (loading control to beta Actin) were diluted to 1/2500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

Predicted band size: 48 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

U937 (Human histiocytic lymphoma cells) whole cell lysate at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Lab

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

False colour image of Western blot : Anti-JNK1 antibody [EPR17557] staining at 1/2500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab199380 was shown to bind specifically to JNK1. A band was observed at 42/48 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in mapk8 knockout cell line ab277181 (knockout cell lysate ab277223). To generate this image, wild-type and mapk8 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

MAPK8 knockout U-2 OS cell lysate at 20 µg

Observed band size: 42-48 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Rat spleen lysate at 10 µg

Lane 4:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 5:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 7:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1:

MDCK (Canine kidney cell line) whole cell lysates at 10 µg

Lane 2:

COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 10 µg

Lane 3:

UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Blocking/Dilution buffer : 5% NFDM/TBST.

Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

Lane 1:

Full length Human JNK1 recombinant protein at 0.01 µg

Lane 2:

Full length Human JNK2 recombinant protein at 0.01 µg

Lane 3:

Full length Human JNK3 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1:

Xenopus (X. tropicalis) muscle lysates at 10 µg

Lane 2:

BHK (Hamster kidney fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)
  • WB

CiteAb

Western blot - Anti-JNK1 antibody [EPR17557] (AB199380)

JNK1 western blot using anti-JNK1 antibody [EPR17557] ab199380. Publication image and figure legend from Zhang, Z., Zhu, L., et al., 2019, Cell Death Dis, PubMed 31296837.

ab199380 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab199380 please see the product overview.

The ERK1/2 signaling pathway was responsible for the effects of CTRP3 on MSCs.a, b Western blot analysis of phospho-Akt/Akt, phospho-ERK1/2/ERK1/2, phospho-AMPK/AMPK, and phospho-JNK/JNK following CTRP3 (n = 3). c, d Effects of U0126 (an ERK1/2 inhibitor) on CTRP3-induced upregulation of MMP9, MT1/2, and SOD2. GAPDH served as a control (n = 4). e–g Effects of ERK1/2 inhibition on MSCs migration and proliferation (n = 4). Images were at original magnification, ×200. (H) CCK-8 assays were used to detect the effects of MMP9 neutralization on CTRP3-induced cell proliferation (n = 5). i, j Effects of ERK1/2 inhibition on apoptosis induced by oxidative stress via TUNEL staining (n = 4). All data are presented as means ± SEMs. *p < 0.05; **p < 0.01; ***p < 0.001

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-JNK1 antibody [EPR17557] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17557

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Chicken, Hamster, Dog, Human, African green monkey, Xenopus tropicalis

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "Hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" }, "Xenopus tropicalis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>" } } }

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 also known as c-Jun N-terminal kinase 1 is a member of the mitogen-activated protein kinase (MAPK) family with a molecular weight of approximately 46 kDa. It is expressed in various tissues throughout the body including the brain heart liver and skeletal muscle. JNK1 exists in multiple isoforms due to alternative splicing. The JNK1 protein is activated by dual phosphorylation on threonine and tyrosine residues a process integral to its function as a stress-activated protein kinase. Commonly used research tools include JNK antibodies and phospho JNK antibodies which help detect the activated forms of this kinase during cellular studies.
Biological function summary

This kinase plays an important role in processes such as inflammation apoptosis and cellular stress responses. JNK1 is not just an isolated enzyme. It forms complexes with other proteins under specific conditions facilitating diverse cellular responses. For example JNK1 activation influences transcription factors like c-Jun by phosphorylating them impacting gene expression related to cell survival and death. This activity establishes JNK1 as a significant player in routine cell functioning and response to external environmental stressors.

Pathways

The kinase is part of the MAPK signaling pathways and the stress-activated protein kinase (SAPK) pathways. These pathways involve multiple signaling cascades important for transmitting extracellular signals into the cellular environment. JNK1 interacts with proteins like MKK4 and MKK7 which are upstream activators and ATF2 a downstream target. This positioning makes JNK1 an essential signaling node that translates extracellular stressors into cellular responses providing adaptability to cells amidst changing conditions.

JNK1 has connections to disorders such as cancer and neurodegenerative diseases. Abnormal activation of JNK1 can lead to irregular cell proliferation making it pertinent in cancer. Similarly in neurodegenerative diseases like Alzheimer's JNK1's involvement in neuronal apoptosis turns critical. It regulates tau phosphorylation connecting JNK1 with tauopathies observed in Alzheimer's disease. Researchers often investigate these associations to understand the mechanisms that underpin these conditions and develop targeted therapies.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
See full target information MAPK8

제품이 사용된 논문 (84)

Recent publications for all applications. Explore the 전체 목록 and refine your search

RSC medicinal chemistry : PubMed40236619

2025

Rutaecarpine derivatives synthesized skeletal reorganization alleviate inflammation-associated oxidative damage by inhibiting the MAPK/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Ying Chen,Cai-Neng Zhang,Xiu-Yun Guo,Liu-Song Lan,Yi-Fan Geng,Jin-Hui Peng,Cheng-Xue Pan,Yan Huang,Gui-Fa Su

Frontiers in oncology 14:1433256 PubMed39717750

2024

Neuroblastoma response to RAS-MAPK inhibitors and APR-246 (eprenetapopt) co-treatment is dependent on SLC7A11.

Applications

Unspecified application

Species

Unspecified reactive species

Vid Mlakar,Ina Oehme,Laurence Lesne,Sara Najafi,Marc Ansari,Fabienne Gumy-Pause

Cell communication and signaling : CCS 22:478 PubMed39375718

2024

Senescent cells promote breast cancer cells motility by secreting GM-CSF and bFGF that activate the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Wang,Yan Fang,Yigong Hou,Dongmei Cheng,Emily V Dressler,Hao Wang,Juan Wang,Guanwen Wang,Yilei Li,Hong Liu,Rong Xiang,Shuang Yang,Peiqing Sun

Chinese medicine 19:128 PubMed39285464

2024

The improvement of modified Si-Miao granule on hepatic insulin resistance and glycogen synthesis in type 2 diabetes mellitus involves the inhibition of TNF-α/JNK1/IRS-2 pathway: network pharmacology, molecular docking, and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Zebiao Cao,Xianzhe Wang,Zhili Zeng,Zhaojun Yang,Yuping Lin,Lu Sun,Qiyun Lu,Guanjie Fan

Cell death & disease 15:395 PubMed38839744

2024

Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Liu,Ke Shao,Wendong Yang,Qi Shen,Mengru Lu,Zhiying Shao,Sufang Chu,Yuming Wang,Xuehao Wang,Xiaofeng Chen,Jin Bai,Xiaofeng Wu

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 28:239-252 PubMed38682172

2024

Dexmedetomidine alleviates blood-brain barrier disruption in rats after cerebral ischemia-reperfusion by suppressing JNK and p38 MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Canmin Zhu,Dili Wang,Chang Chang,Aofei Liu,Ji Zhou,Ting Yang,Yuanfeng Jiang,Xia Li,Weijian Jiang

Naunyn-Schmiedeberg's archives of pharmacology 397:6611-6621 PubMed38472369

2024

Morroniside improves AngII-induced cardiac fibroblast proliferation, migration, and extracellular matrix deposition by blocking p38/JNK signaling pathway through the downregulation of KLF5.

Applications

Unspecified application

Species

Unspecified reactive species

Haotian Zheng,Linxin Yang,Huashang Huang,Yazhou Lin,Lin Chen

Discover oncology 15:45 PubMed38383815

2024

TRAIL predisposes non-small cell lung cancer to ferroptosis by regulating ASK-1/JNK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Liu,Huiqian Deng,Mi Huang,Wei Zhou,Yilin Yang

Experimental and therapeutic medicine 27:55 PubMed38234627

2024

PDCD4 silencing alleviates KA‑induced neurotoxicity of HT22 cells by inhibiting endoplasmic reticulum stress via blocking the MAPK/NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Guiling Cao

Experimental and therapeutic medicine 27:8 PubMed38223326

2024

Butorphanol inhibits ferroptosis to attenuate PC12 cell injury by blocking JNK/p38 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Ji,Qing She,Ping Zhou,Yibin Qin
제품이 사용된 논문 모두 보기

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