AB52647

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]

Name: Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]
Brand: Abcam Limited
SKU: AB52647
Rating: 2 (3 reviews)

20ul selling size
RabMAb
Recombinant
KO Validated
각 태그의 뜻

(3 리뷰들)

(27 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Junctional Adhesion Molecule 1/JAM-A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 27 publications.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

Immunohistochemical staining of paraffin-embedded human liver using unpurified ab52647 at a 1/100 dilution.

Lab

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647) at 1/1000 dilution

All lanes:

TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 32 kDa

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Supplier Data

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

Lane 1 : Wild-type HAP1 whole cell lysate (40 μg)
Lane 2 : Junctional Adhesion Molecule 1/JAM-A knockout HAP1 whole cell lysate (40 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab52647 observed at 32 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab52647 was shown to specifically react with Junctional Adhesion Molecule 1/JAM-A in wild-type HAP1 cells as signal was lost in Junctional Adhesion Molecule 1 knockout cells. Wild-type and Junctional Adhesion Molecule 1/JAM-A knockout samples were subjected to SDS-PAGE. ab52647 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

Predicted band size: 32 kDa

false

Unknown

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647) at 1/2000 dilution

All lanes:

Hela cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 32 kDa

Observed band size: 33 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1042Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.

Biological function summary

JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.

Pathways

JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.

JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed : 11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed : 11812992). Involved in platelet activation (PubMed : 10753840).. (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.. (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.

See full target information F11R

대체 명칭 보기

CD321, JAM1, JCAM, UNQ264/PRO301, F11R, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, JAM-1, PAM-1

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제품이 사용된 논문 (27)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell communication and signaling : CCS 22:578 PubMed39627817

2024

Cultivation and molecular characterization of viable Helicobacter pylori from the root canal of 170 deciduous teeth of children.

Applications

Unspecified application

Species

Unspecified reactive species

Wieland Elger,Nicole Tegtmeyer,Manfred Rohde,Bodo Linz,Christian Hirsch,Steffen Backert

Frontiers in bioengineering and biotechnology 10:958072 PubMed35992356

2022

Improvement of the bladder perfusion curative effect through tight junction protein degradation induced by magnetic temperature-sensitive hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Sun,Xinhong Song,Peng Guo,Dong Zhang,Shishuai Zuo,Kang Leng,Yun Liu,Haiyang Zhang

Evidence-based complementary and alternative medicine : eCAM 2021:5792130 PubMed34795785

2021

Mechanism of QingHuaZhiXie Prescription Regulating TLR4-IECs Pathway in the Intervention of Diarrhea Predominant Irritable Bowel Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Huang,Ping Zhao,Meijuan Xi,Fang Li,Lijiang Ji

CNS neuroscience & therapeutics : PubMed33987940

2021

Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Jian-Jun Lv,Yu Zhao,Hao-Lin Wei,Tian-Jiao Zhang,Hai-Jiao Yang,Zhi-Nan Chen,Jian-Li Jiang

Gut microbes 13:1-21 PubMed33970782

2021

PqqE is a new virulence factor that cleaves junctional adhesion molecule A and disrupts gastric epithelial integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel S Marques,Ana C Costa,Hugo Osório,Marta L Pinto,Sandra Relvas,Mário Dinis-Ribeiro,Fátima Carneiro,Marina Leite,Ceu Figueiredo

Cancer science 112:906-917 PubMed33185939

2020

Aberrant expression of junctional adhesion molecule-A contributes to the malignancy of cervical adenocarcinoma by interaction with poliovirus receptor/CD155.

Applications

Unspecified application

Species

Unspecified reactive species

Taro Murakami,Akira Takasawa,Kumi Takasawa,Taishi Akimoto,Tomoyuki Aoyama,Kazufumi Magara,Yuki Saito,Misaki Ota,Daisuke Kyuno,Soh Yamamoto,Tadashi Hasegawa,Tsuyoshi Saito,Makoto Osanai

Frontiers in neurology 11:887 PubMed32973660

2020

Genetic Deletion or Pharmacological Inhibition of Cyclooxygenase-2 Reduces Blood-Brain Barrier Damage in Experimental Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Changjun Yang,Yi Yang,Kelly M DeMars,Gary A Rosenberg,Eduardo Candelario-Jalil

Journal of cellular biochemistry 121:1703-1715 PubMed31595574

2019

SNP rs710886 A>G in long noncoding RNA PCAT1 is associated with the risk of endometriosis by modulating expression of multiple stemness-related genes via microRNA-145 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Wang,Qi Xing,Tongfu Feng,Ming He,Weixu Yu,Hui Chen

Cell biochemistry and function 37:72-83 PubMed30773657

2019

Proteomic analysis of the effects of cell culture density on the metastasis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Man-Lan Guo,Mi-Xin Sun,Jin-Zhi Lan,Li-Sha Yan,Jing-Juan Zhang,Xiao-Xia Hu,Shu Xu,Da-Hua Mao,Hai-Song Yang,Ya-Wei Liu,Teng-Xiang Chen

Traffic (Copenhagen, Denmark) 19:879-892 PubMed30095213

2018

Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase.

Applications

Unspecified application

Species

Unspecified reactive species

Cameron Schlegel,Lynne A Lapierre,Victoria G Weis,Janice A Williams,Izumi Kaji,Carolina Pinzon-Guzman,Nripesh Prasad,Braden Boone,Angela Jones,Hernan Correa,Shawn E Levy,Xianlin Han,Miao Wang,Kelly Thomsen,Sari Acra,James R Goldenring

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (AB52647)

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (27)

Product promise