AB238313

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal

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Rabbit Monoclonal Junctional Adhesion Molecule 1/JAM-A antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human F11R.

대체 명칭 보기

CD321, JAM1, JCAM, UNQ264/PRO301, F11R, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, JAM-1, PAM-1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (AB238313)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (AB238313)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Junctional Adhesion Molecule 1/JAM-A using ab238313 at 1/10,0000 dilution in immunohistochemical analysis.

Lab

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (AB238313)

Western blot : Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal ab238313 staining at 1/250 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 19 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in F11R knockout U-87 MG cell line (ab326032). To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (ab238313) at 1/250 dilution

Lane 1:

Wild-type U-87 MG whole cell lysate at 20 µg

Lane 2:

Western blot - Human F11R knockout U-87 MG cell line (<a href='/ko/products/cell-lines/human-f11r-knockout-u-87-mg-cell-line-ab326032'>ab326032</a>) at 20 µg

Lane 3:

DU 145 whole cell lysate at 20 µg

Lane 4:

Saos-2 whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa

false

Supplier Data

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (AB238313)

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (ab238313) at 1/250 dilution

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 15 kDa,32 kDa,344 kDa,52 kDa,55 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM275

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human F11R. The exact immunogen used to generate this antibody is proprietary information.

Q9Y624

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000 - 1/10000", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from an animal origin–free culture supernatant.

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.

Biological function summary

JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.

Pathways

JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.

JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed : 11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed : 11812992). Involved in platelet activation (PubMed : 10753840).. (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.. (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.

See full target information F11R

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cachexia, sarcopenia and muscle 16:e13734 PubMed39971710

2025

SIRT6 Ameliorates Cancer Cachexia-Associated Adipose Wasting by Suppressing TNFR2 Signalling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Xu,Yida Wang,Fang Wang,Yannan Guo,Yu Ren,Vivien Low,Sungyun Cho,Qingfei Liu,Ying Qiu,Xue Li,Kang Yu,Zhongchi Li,Zhao Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (AB238313)

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (AB238313)

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [RM275] - N-terminal (AB238313)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise