AB12723

Anti-Kaiso antibody [6F / 6F8]

Name: Anti-Kaiso antibody [6F / 6F8]
Brand: Abcam Limited
SKU: AB12723
Rating: 5 (5 reviews)

(5 리뷰들)

(23 제품이 사용된 논문 )

Mouse Monoclonal KAISO antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse samples. Cited in 23 publications.

5 이미지

Flow Cytometry (Intracellular) - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (AB12723)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.

Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Immunofluorescence using clone 6F / 6F8 on C2C12 cells.

PubMed

Western blot - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Representative western blot detecting Kaiso in ARPE-19 cells using ab12723.

All lanes:

Western blot - Anti-Kaiso antibody [6F / 6F8] (ab12723)

Predicted band size: 74 kDa

false

Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.

Unknown

Western blot - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.

All lanes:

Western blot - Anti-Kaiso antibody [6F / 6F8] (ab12723)

Predicted band size: 74 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

6F / 6F8

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

We can conjugate this antibody to FITC for you (please see ab150256 for details).

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Kaiso also known as ZBTB33 is a transcriptional regulator with a mass of approximately 75 kilodaltons. It is a member of the POZ-zinc finger family of proteins. Researchers identified Kaiso as a dual-specificity transcription factor meaning it can bind to both DNA methylated and non-methylated sequences. It is widely expressed in various tissues including the brain liver heart and testis. This broad expression pattern indicates its potential involvement in multiple cellular processes.

Biological function summary

Kaiso functions as a transcriptional repressor influencing gene expression by binding to specific DNA sequences. It operates as part of a protein complex that includes other corepressors like N-CoR and SMRT. These interactions modulate chromatin structure which affects gene accessibility. Kaiso's repression of target genes is essential for maintaining cellular homeostasis. Research shows that Kaiso also interacts with p120-catenin a protein involved in cell adhesion linking its function to cytoskeletal dynamics and cell motility.

Pathways

Several studies show that Kaiso is part of key signaling cascades such as the Wnt/β-catenin pathway. It interacts with other pathway regulators including TCF/LEF transcription factors and is influenced by β-catenin signaling which plays an important role in embryonic development and cellular proliferation. Another significant pathway involving Kaiso is the Notch signaling pathway where Kaiso's function contributes to cell fate determination and differentiation. Through these pathways its ability to regulate gene expression impacts many biological processes.

Kaiso has been linked to cancer progression and metastasis. Its downregulation or loss of function can lead to tumor growth primarily due to the deregulation of genes involved in cell proliferation and apoptosis. Studies highlight its association with colorectal cancer where it influences the expression of tumor suppressor genes. Additionally Kaiso's interaction with β-catenin connects it to other diseases like colorectal cancer where dysregulation of the Wnt pathway plays a critical role. These connections emphasize the potential impact of Kaiso on disease pathogenesis and its value as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed : 23251453).

See full target information ZBTB33

대체 명칭 보기

KAISO, ZNF348, ZBTB33, Transcriptional regulator Kaiso, Zinc finger and BTB domain-containing protein 33

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제품이 사용된 논문 (23)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta neuropathologica communications 12:72 PubMed38711090

2024

Comprehensive analysis of the REST transcription factor regulatory networks in IDH mutant and IDH wild-type glioma cell lines and tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Malgorzata Perycz,Michal J Dabrowski,Marta Jardanowska-Kotuniak,Adria-Jaume Roura,Bartlomiej Gielniewski,Karolina Stepniak,Michał Dramiński,Iwona A Ciechomska,Bozena Kaminska,Bartosz Wojtas

The ocular surface 29:301-310 PubMed37268293

2023

Manufacturing of human corneal endothelial grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ting Zhu,Sean Tighe,Shuang-Ling Chen,Yuan Zhang,Szu-Yu Chen,Winston W Y Kao,Scheffer C G Tseng

Cancers 15: PubMed37190208

2023

Immune Profile of Exosomes in African American Breast Cancer Patients Is Mediated by Kaiso/THBS1/CD47 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Md Shakir Uddin Ahmed,Brittany D Lord,Benjamin Adu Addai,Sandeep K Singhal,Kevin Gardner,Ahmad Bin Salam,Anghesom Ghebremedhin,Jason White,Iqbal Mahmud,Rachel Martini,Deepa Bedi,Huixian Lin,Jacqueline D Jones,Balasubramanyanam Karanam,Windy Dean-Colomb,William Grizzle,Honghe Wang,Melissa Davis,Clayton C Yates

Journal of Taibah University Medical Sciences 18:802-811 PubMed36852243

2023

Association of Kaiso and partner proteins in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hira Salam,Shaheen Ahmed,Muhammad Furqan Bari,Uzma Bukhari,Ghulam Haider,Shariq Najeeb,Nouman Mughal

International journal of molecular medicine 47: PubMed33576467

2021

Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Tong,Jia Li,Xinzhe Feng,Chen Wang,Yihong Xu,Chongru He,Weidong Xu

Communications biology 4:150 PubMed33526872

2021

Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survival.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep K Singhal,Jung S Byun,Samson Park,Tingfen Yan,Ryan Yancey,Ambar Caban,Sara Gil Hernandez,Stephen M Hewitt,Heike Boisvert,Stephanie Hennek,Mark Bobrow,Md Shakir Uddin Ahmed,Jason White,Clayton Yates,Andrew Aukerman,Rami Vanguri,Rohan Bareja,Romina Lenci,Paula Lucia Farré,Adriana De Siervi,Anna María Nápoles,Nasreen Vohra,Kevin Gardner

The Journal of biological chemistry 294:12957-12974 PubMed31296660

2019

Temporal and differential regulation of KAISO-controlled transcription by phosphorylated and acetylated p53 highlights a crucial regulatory role of apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Seo-Hyun Choi,Dong-In Koh,Su-Yeon Cho,Min-Kyeong Kim,Kyung-Sup Kim,Man-Wook Hur

Scientific reports 9:3054 PubMed30816218

2019

Regulation of Cathepsin E gene expression by the transcription factor Kaiso in MRL/lpr mice derived CD4+ T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sumie Hiramatsu,Katsue S Watanabe,Sonia Zeggar,Yosuke Asano,Yoshia Miyawaki,Yuriko Yamamura,Eri Katsuyama,Takayuki Katsuyama,Haruki Watanabe,Mariko Takano-Narazaki,Yoshinori Matsumoto,Tomoko Kawabata,Ken-Ei Sada,Jun Wada

Cancer letters 431:1-10 PubMed29751044

2018

Transcriptional repressor Kaiso promotes epithelial to mesenchymal transition and metastasis in prostate cancer through direct regulation of miR-200c.

Applications

ICC/IF

Species

Unspecified reactive species

Abisola Abisoye-Ogunniyan,Huxian Lin,Anghesom Ghebremedhin,Ahmad Bin Salam,Balasubramanyam Karanam,Shaniece Theodore,Jacqueline Jones-Trich,Melissa Davis,William Grizzle,Honghe Wang,Clayton Yates

Scientific reports 7:7116 PubMed28769046

2017

Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members.

Applications

WB, ChIP, EMSA, ICC/IF

Species

Human, Human, Human, Human

Xiaodong Xue,Jian Zhang,Huai Lan,Yinli Xu,Huishan Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kaiso antibody [6F / 6F8]

Flow Cytometry (Intracellular) - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Western blot - Anti-Kaiso antibody [6F / 6F8] (AB12723)

Western blot - Anti-Kaiso antibody [6F / 6F8] (AB12723)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (23)

Product promise