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AB254258

Anti-Kallikrein 7/KLK7 antibody [EPR22594-203]

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Kallikrein 7/KLK7 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

대체 명칭 보기

PRSS6, SCCE, KLK7, Kallikrein-7, hK7, Serine protease 6, Stratum corneum chymotryptic enzyme, hSCCE

6 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)

Immunohistochemical analysis of paraffin-embedded human skin tissue labelling KLK7 with ab254258 at 1/500 dilution for 30 minutes at room temperature, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stratum corneum of human skin (PMID 23999494). Hematoxylin used as a counterstain.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)

Immunohistochemical analysis of 0.2% Triton X-100 permeabilised, 4% PFA fixed mouse skin tissue labelling KLK7 with ab254258 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Specific staining on stratum corneum of mouse skin (PMID : 23999494).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labelling KLK7 with ab254258 at 1/500 dilution for 30 minutes at room temperature, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stratum corneum of rat skin (PMID 23999494). Hematoxylin used as a counterstain.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)

Immunohistochemical analysis of 0.2% Triton X-100 permeabilised, 4% PFA fixed rat skin tissue labelling KLK7 with ab254258 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Specific staining on stratum corneum of rat skin (PMID : 23999494).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labelling KLK7 with ab254258 at 1/500 dilution for 30 minutes at room temperature, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stratum corneum of mouse skin (PMID 23999494). Hematoxylin used as a counterstain.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Western blot - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)
  • WB

Unknown

Western blot - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (AB254258)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258) at 1/1000 dilution

Lane 1:

Mouse skin lysate at 20 µg

Lane 2:

Rat skin lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22594-203

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB244367

Anti-Kallikrein 7/KLK7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 7/KLK7 antibody (AB244367)

  • 0
  • 0
Primary Antibodies

AB200745

Anti-Kallikrein 7/KLK7 antibody

Western blot - Anti-Kallikrein 7/KLK7 antibody (AB200745)

  • 0
  • 0
Primary Antibodies

AB96710

Anti-Kallikrein 7/KLK7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody (AB96710)

  • 0
  • 0

보완 제품

Assay Kits

AB287175

Human Kallikrein 7/KLK7 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB176299

Anti-Kallikrein 5 antibody

Western blot - Anti-Kallikrein 5 antibody (AB176299)

  • 0
  • 0
Primary Antibodies

AB221155

Anti-Filaggrin antibody [EPR21892]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [EPR21892] (AB221155)

  • 5
  • 3
Primary Antibodies

AB209490

Anti-Desmoglein 1/DSG1 antibody [EPR20382]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 7 also known as KLK7 is a serine protease enzyme active in the skin. The protein's mass is approximately 27 kDa. It helps in the desquamation process by breaking down corneodesmosomes which are cell structures that facilitate cell adhesion in the outer skin layer. Kallikrein 7 is highly expressed in the epidermis and various mucosal surfaces reflecting its essential role in maintaining skin barrier functions.
Biological function summary

KLK7 contributes to skin homeostasis and renewal processes. Its enzymatic action involves cleaving proteins like desmoglein and corneodesmosin which are part of the desmosomal complex. This activity promotes the natural shedding of skin cells a process important for skin turnover and repair. By regulating this balance KLK7 supports not only skin health but also its resilience against external stressors.

Pathways

KLK7 plays a significant role in processes such as the proteolytic cascade and inflammation. It is involved in the kallikrein-kinin system alongside proteins like KLK5 that modulate inflammatory responses and barrier function in skin. KLK7 also interacts with pathways related to tissue remodeling influencing processes like inflammation through cytokine activation.

KLK7 is linked to skin conditions like atopic dermatitis and psoriasis. These disorders often feature a disrupted skin barrier where KLK7-mediated proteolytic activity increases and may exacerbate inflammation. The relationship with inflammation also connects KLK7 to proteins such as KLK5 which can further intensify skin disorders when their activities are unregulated.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
See full target information KLK7
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular neurobiology 62:14261-14285 PubMed40681826

2025

Reduced Availability of Essential Amino Acids Disrupts Differentiation of Anorexigenic POMC Neurons in the Fetal Rat Hypothalamus.

Applications

Unspecified application

Species

Unspecified reactive species

Pieter Vancamp,Isabelle Grit,Marie Demonceaux,Véronique Ferchaud-Roucher,Patricia Parnet,Valérie Amarger

BMC cancer 24:794 PubMed38961454

2024

KLK7 expression in human tumors: a tissue microarray study on 13,447 tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Kind,Carolina Palacios Castillo,Ria Schlichter,Natalia Gorbokon,Maximilian Lennartz,Lisa S Hornsteiner,Sebastian Dwertmann Rico,Viktor Reiswich,Florian Viehweger,Martina Kluth,Claudia Hube-Magg,Christian Bernreuther,Franziska Büscheck,Till S Clauditz,Christoph Fraune,Andrea Hinsch,Till Krech,Patrick Lebok,Stefan Steurer,Eike Burandt,Sarah Minner,Andreas H Marx,Ronald Simon,Waldemar Wilczak,Guido Sauter,Anne Menz,Frank Jacobsen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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