AB248964

Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal Kallikrein 8/KLK8 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

NRPN, PRSS19, TADG14, UNQ283/PRO322, KLK8, Kallikrein-8, hK8, Neuropsin, Ovasin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein, NP

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free (AB248964)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free (AB248964)

This data was developed using ab150395, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling Kallikrein 8/KLK8 with ab150395 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free (AB248964)

This data was developed using ab150395, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (<a href='/ko/products/primary-antibodies/kallikrein-8-klk8-antibody-epr57522-ab150395'>ab150395</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Human fetal brain cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit

Predicted band size: 28 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5752(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab248964 is the carrier-free version of ab150395.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 8 also known as KLK8 is a serine protease enzyme with a molecular mass of approximately 28 kDa. Alternative names include Neurosin and hK8. It is expressed in various tissues notably in the brain testis and skin. KLK8 plays a mechanical role in cleaving protein substrates in these regions aiding in maintaining cellular structures and processes through its proteolytic activity. Its expression is important in neuronal development making it significant in central nervous system function.

Biological function summary

KLK8 involves itself in tissue remodeling and neural plasticity. It interacts with extracellular matrix proteins contributing to cell migration and adhesion. Important in synaptic plasticity KLK8 supports memory formation and learning. KLK8 operates as part of proteolytic cascades and may overlap with other kallikreins in function. Its activity includes modulating neural and skin cells suggesting its importance in cellular differentiation and growth.

Pathways

KLK8 takes part in the kallikrein-kinin system and extracellular matrix degradation pathways. It interacts with proteins such as KLK3 (prostate-specific antigen) within these pathways although KLK3 is more associated with prostate tissue. KLK8's function in these pathways ensures proper maintenance and remodeling of tissue-specific extracellular environments. These activities highlight its relevance in maintaining physiological balance and cellular responses in its primary tissues of expression.

KLK8 links with neurological conditions and certain skin disorders. Altered levels or dysfunctions of KLK8 correlate with disorders like Alzheimer's disease indicating its potential role in amyloid processing. Similar associations exist with skin disorders like psoriasis where KLK8 influences inflammatory response. Research also explores its connection with the protein amyloid-beta contributing to an understanding of its involvement in neurodegenerative diseases. Understanding these interactions can further illuminate potential therapeutic targets for treating these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.

See full target information KLK8

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free (AB248964)

Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free (AB248964)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise