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AB243870

Anti-KAP1 (phospho S824) antibody [BL-246-7B5]

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(3 제품이 사용된 논문 )

Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (ab243870) is a rabbit monoclonal antibody detecting KAP1 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Recombinant format for unrivalled batch-batch consistency

대체 명칭 보기

KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1

7 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue and serial sections of etoposide treated HeLa cells stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right) are also shown. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with etoposide (right) or untreated (left) labeling KAP-1 (phospho S824) (red) using ab243862 at 1/100 dilution in ICC analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstain : Phalloidin conjugated Alexa Fluor® 488 (green).

Flow Cytometry - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

Flow cytometry overlay histogram of 4% formaldehyde fixed HEK293T cells permeabilized with 90% methanol labeling human Phospho KAP-1 (S824) with ab243870 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded human prostate carcinoma tissue labeling human Phospho KAP-1 (S824) with ab243870, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on human prostate carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)
  • IP

Unknown

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

KAP1 (phospho S824) was immunoprecipitated from 1 mg HEK-293T whole cell lysate treated with 100 µM etoposide or untreated, using ab243870 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243870 at 1/1000 dilution. Lane 1 : ab243870 IP in HEK-293T cell lysate treated with 100 µM etoposide.

Lane 2 : ab243870 Ip in untreated HEK-293T whole cell lysate.

Lane 3 : Control IgG in HEK-293T whole cell lysate treated with 100 µM etoposide.

Detection : Chemiluminescence with an exposure time of 10 seconds

All lanes:

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (ab243870)

Predicted band size: 88 kDa

false

Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)
  • WB

Supplier Data

Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

All lanes:

Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (ab243870) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) cells treated with 100 µM etoposide (+) at 50 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) cells, mock treated (-) at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Exposure time: 30s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Unconjugated

    Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-246-7B5

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt, IP, ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TRIM28 pS824. The exact immunogen used to generate this antibody is proprietary information.

Q13263

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대체품

Primary Antibodies

AB3831

Anti-KAP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (AB3831)

  • 5
  • 4
Primary Antibodies

AB22553

Anti-KAP1 antibody [20C1]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [20C1] (AB22553)

  • 4
  • 14
Primary Antibodies

AB10483

Anti-KAP1 antibody

Immunoprecipitation - Anti-KAP1 antibody (AB10483)

  • 3
  • 7
Primary Antibodies

AB10484

Anti-KAP1 antibody

Western blot - Anti-KAP1 antibody (AB10484)

  • 5
  • 6
Primary Antibodies

AB243871

Anti-KAP1 antibody [BL-248-2G6]

Recombinant

Immunocytochemistry - Anti-KAP1 antibody [BL-248-2G6] (AB243871)

  • 0
  • 0
Primary Antibodies

AB109545

Anti-KAP1 antibody [EPR5249]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-KAP1 antibody [EPR5249] (AB109545)

  • 2
  • 1
Primary Antibodies

AB109287

Anti-KAP1 antibody [EPR5216]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (AB109287)

  • 5
  • 4
Primary Antibodies

AB109289

Anti-KAP1 antibody [EPR5217]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5217] (AB109289)

  • 0
  • 0
Primary Antibodies

AB133440

Anti-KAP1 (phospho S824) antibody [EPR5248]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440)

  • 5
  • 1
Primary Antibodies

AB215549

Anti-KAP1 (phospho S824) antibody [EPR5248] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] - BSA and Azide free (AB215549)

  • 0
  • 0
Primary Antibodies

AB215550

Anti-KAP1 antibody [EPR5217] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5217] - BSA and Azide free (AB215550)

  • 0
  • 0
Primary Antibodies

AB84077

Anti-KAP1 (phospho S824) antibody

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody (AB84077)

  • 0
  • 0
Primary Antibodies

AB70369

Anti-KAP1 (phospho S824) antibody

Immunocytochemistry - Anti-KAP1 (phospho S824) antibody (AB70369)

  • 5
  • 6
Primary Antibodies

AB190178

Anti-KAP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody (AB190178)

  • 0
  • 0

보완 제품

Primary Antibodies

AB109287

Anti-KAP1 antibody [EPR5216]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (AB109287)

  • 5
  • 4
Primary Antibodies

AB81292

Anti-ATM (phospho S1981) antibody [EP1890Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)

  • 5
  • 18
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB32420

Anti-ATM antibody [Y170]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM antibody [Y170] (AB32420)

  • 5
  • 8
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p>Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 20μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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제품 세부 정보

What is this antibody validated in?
Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (ab243870) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Other related products
We have a range of other formats of antibody clone [BL-246-7B5] also available for your convenience: ab243870, ab272068

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Recombinant antibody was purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KAP1 also known as TRIM28 or TIF1Β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.
Biological function summary

KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.

Pathways

KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.

KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also a corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteasomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed : 23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610).. (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.
See full target information TRIM28 pS824
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of orthopaedic surgery and research 20:336 PubMed40176111

2025

USP15-modified ADMSCs-Exo alleviates chondrocyte damage and effectively relieved osteoarthritis by inducing M2 polarization of macrophages through deubiquitinating FOXC1.

Applications

Unspecified application

Species

Unspecified reactive species

Qibin Liang,Qinghe Ding,Liang Zhao,Jingchao Tan,Wei Niu

Nature communications 14:5147 PubMed37620345

2023

RNAPII-dependent ATM signaling at collisions with replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Einig,Chao Jin,Valentina Andrioletti,Boris Macek,Nikita Popov

Nature medicine 29:1400-1411 PubMed37277454

2023

Camonsertib in DNA damage response-deficient advanced solid tumors: phase 1 trial results.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy A Yap,Elisa Fontana,Elizabeth K Lee,David R Spigel,Martin Højgaard,Stephanie Lheureux,Niharika B Mettu,Benedito A Carneiro,Louise Carter,Ruth Plummer,Gregory M Cote,Funda Meric-Bernstam,Joseph O'Connell,Joseph D Schonhoft,Marisa Wainszelbaum,Adrian J Fretland,Peter Manley,Yi Xu,Danielle Ulanet,Victoria Rimkunas,Mike Zinda,Maria Koehler,Ian M Silverman,Jorge S Reis-Filho,Ezra Rosen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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