AB10308

Anti-KAT13A / SRC1 antibody

Name: Anti-KAT13A / SRC1 antibody
Brand: Abcam Limited
SKU: AB10308
Rating: 5 (3 reviews)

(3 리뷰들)

(1 출판물)

Rabbit Polyclonal KAT13A / SRC1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NCOA1 aa 1400 to C-terminus.

대체 명칭 보기

BHLHE74, SRC1, NCOA1, Nuclear receptor coactivator 1, NCoA-1, Class E basic helix-loop-helix protein 74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, bHLHe74, SRC-1

2 이미지

Supplier Data

Immunoprecipitation - Anti-KAT13A / SRC1 antibody (AB10308)

Detection of KAT13A / SRC1 by western blot of immunoprecipitates.
Samples : Lane 1 and 2 : Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells prepared using NETN lysis buffer. Lane 3 : Control IgG.
Antibodies : ab10308 (lot 2, lane 2) used for IP at 6 µg per reaction. KAT13A / SRC1 was also immunoprecipitated by a previous lot of this antibody (lot 1, lane 1). For blotting immunoprecipitated KAT13A / SRC1, ab10308 was used at 0.4 µg/mL.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-KAT13A / SRC1 antibody (ab10308)

Predicted band size: 156 kDa

false

Supplier Data

Western blot - Anti-KAT13A / SRC1 antibody (AB10308)

All lanes:

Western blot - Anti-KAT13A / SRC1 antibody (ab10308) at 0.1 µg/mL

Lane 1:

HEK293T whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 15 µg

Lane 3:

HEK293T whole cell lysate at 5 µg

Lane 4:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 156 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NCOA1 aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15788

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance of 1.4 at 280 nm equals 1.0 mg of IgG.

보관 버퍼

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KAT13A also known as SRC1 or nuclear receptor coactivator 1 (NCOA1) is a coactivator protein with an approximate mass of 160 kDa. This protein aids in the function of nuclear receptors and exerts its mechanical roles by interacting with transcription factors facilitating transcriptional activation. KAT13A is widely expressed in tissues such as the liver skeletal muscle and brain highlighting its ubiquitous role in cellular processes.

Biological function summary

KAT13A modulates gene expression by forming part of multi-protein complexes significantly affecting cellular functions like growth and differentiation. The protein serves as a bridge between nuclear receptors and the transcriptional machinery effectively enhancing transcription upon binding. It also plays a role in steroid hormone responses impacting pathways involved in metabolism and inflammation.

Pathways

KAT13A participates in the steroid hormone receptor signaling pathway and the PPAR signaling pathway. Within these pathways it interacts with proteins such as the estrogen receptor and PPAR-alpha contributing to energy homeostasis and regulating lipid metabolism. These interactions position KAT13A as an important player in integrating hormonal signals with transcriptional response.

KAT13A's dysregulation associates with cancer particularly breast cancer and metabolic disorders like obesity. Its relationship with the estrogen receptor links KAT13A to hormone-dependent cancers affecting cancer cell proliferation. Additionally changes in PPAR-alpha interaction relate to metabolic syndrome influencing lipid metabolism and contributing to the development of obesity-related complications.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

See full target information NCOA1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of endocrinology 237:255-269 PubMed29636364

2018

Bufalin suppresses endometriosis progression by inducing pyroptosis and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon Jean Cho,Jiyeun E Lee,Mi Jin Park,Bert W O'Malley,Sang Jun Han

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT13A / SRC1 antibody

Immunoprecipitation - Anti-KAT13A / SRC1 antibody (AB10308)

Western blot - Anti-KAT13A / SRC1 antibody (AB10308)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise