AB10508

Anti-KAT13C / NCOA2 antibody

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Polyclonal KAT13C / NCOA2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NCOA2 aa 1400 to C-terminus.

대체 명칭 보기

BHLHE75, SRC2, TIF2, NCOA2, Nuclear receptor coactivator 2, NCoA-2, Class E basic helix-loop-helix protein 75, Transcriptional intermediary factor 2, bHLHe75, hTIF2

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13C / NCOA2 antibody (AB10508)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13C / NCOA2 antibody (AB10508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling KAT13C / NCOA2 with ab10508 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-KAT13C / NCOA2 antibody (AB10508)

ab10508 immunoprecipitation of human NCOA2 (SRC2).

Samples : Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells.

Antibody : ab10508 used at 20 μg/10 mg lysate.

Detection : Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2).

All lanes:

Immunoprecipitation - Anti-KAT13C / NCOA2 antibody (ab10508)

Predicted band size: 159 kDa

false

Supplier Data

Western blot - Anti-KAT13C / NCOA2 antibody (AB10508)

All lanes:

Western blot - Anti-KAT13C / NCOA2 antibody (ab10508) at 1 µg/mL

Lane 1:

TCMK-1 whole cell lysate at 50 µg

Lane 2:

4T1 whole cell lysate at 50 µg

Lane 3:

CT26.WT whole cell lysate at 50 µg

Predicted band size: 159 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human NCOA2 aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15596

Specificity

We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibodies were affinity purified using the peptide immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Do Not Freeze

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KAT13C also known as NCOA2 functions as a transcriptional coactivator involved in nuclear receptor-mediated gene expression. It has a molecular mass of approximately 170 kDa. This protein localizes in the nucleus where it binds with nuclear receptors to enhance transcriptional programming. It is expressed in various tissues including the brain heart and liver indicating its involvement in a diverse range of cellular activities.

Biological function summary

KAT13C/NCOA2 serves as part of a larger protein complex that regulates the expression of target genes following ligand binding to nuclear receptors. It acts as a scaffold protein recruiting additional transcriptional machinery to ensure efficient transcriptional activation. This role is critical for the regulation of hormone-responsive genes and in metabolism cell proliferation and differentiation processes.

Pathways

KAT13C/NCOA2 plays a significant role in the steroid hormone receptor signaling pathway and the nuclear hormone receptor signaling pathway. It interacts with other proteins like p160 family members and the CREB-binding protein (CBP) facilitating coordinated gene expression involved in hormone response and metabolism. These interactions position KAT13C/NCOA2 as an important player in fine-tuning cellular responses to external hormonal signals.

KAT13C/NCOA2 associates with prostate cancer and acute myeloid leukemia. In prostate cancer its interaction with androgen receptor signaling enhances cell proliferation. Meanwhile KAT13C/NCOA2 amplifies gene expression involved in leukemia progression through its interaction with MLL fusion proteins which are implicated in the development of acute myeloid leukemia. These connections underline its role in disease states where abnormal signaling and transcriptional regulation occur.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed : 23508108, PubMed : 8670870, PubMed : 9430642, PubMed : 22504882, PubMed : 26553876). Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1) (PubMed : 23508108, PubMed : 8670870, PubMed : 9430642). Required with NCOA1 to control energy balance between white and brown adipose tissues (PubMed : 23508108, PubMed : 8670870, PubMed : 9430642). Critical regulator of glucose metabolism regulation, acts as a RORA coactivator to specifically modulate G6PC1 expression (PubMed : 23508108, PubMed : 8670870, PubMed : 9430642). Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3 (PubMed : 23508108). Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-BMAL1 heterodimer (By similarity).

See full target information NCOA2

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 42:112013 PubMed36656711

2023

VGLL2-NCOA2 leverages developmental programs for pediatric sarcomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Watson,Collette A LaVigne,Lin Xu,Didier Surdez,Joanna Cyrta,Delia Calderon,Matthew V Cannon,Matthew R Kent,Katherine M Silvius,Jack P Kucinski,Emma N Harrison,Whitney Murchison,Dinesh Rakheja,Franck Tirode,Olivier Delattre,James F Amatruda,Genevieve C Kendall

PloS one 6:e22426 PubMed21829460

2011

A systems biology-based classifier for hepatocellular carcinoma diagnosis.

Applications

IHC-P

Species

Human

Yanqiong Zhang,Shaochuang Wang,Dong Li,Jiyang Zhnag,Dianhua Gu,Yunping Zhu,Fuchu He

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT13C / NCOA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13C / NCOA2 antibody (AB10508)

Immunoprecipitation - Anti-KAT13C / NCOA2 antibody (AB10508)

Western blot - Anti-KAT13C / NCOA2 antibody (AB10508)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise